PXD015006

PXD015006 is an original dataset announced via ProteomeXchange.

Title	Stroke Recovery Dominated by Dysregulation of Complement System – A Longitudinal Proteomics Pathway Analysis
Description	Post-stroke recovery is an ongoing process as the brain attempts to reverse or minimize ischemia- induced disturbances in multiple biological systems. Currently, the longitudinal profile of molecular changes in patients post-stroke is relatively unknown. We therefore aimed to characterise biological pathways in plasma involved in post-stroke recovery using a discovery proteomics workflow coupled with a topological pathway systems biology approach. Blood samples (n = 180, EDTA plasma) were taken from a subgroup of 60 first episode stroke survivors from the Australian START cohort over 3 timepoints: 3-7 days (T1), 3-months (T2) and 12-months (T3) post primary event . Samples were analysed by liquid chromatography mass spectrometry (LC-MS) on the Q Exactive HF hybrid quadrupole-Orbitrap (Thermo-Fisher Scientific) using label-free quantification. The proteomics results were analysed for differential expression of all identified proteins for all available pairwise time-point comparisons. The resulting significant lists of proteins were then submitted for Gene Graph Enrichment Analysis (GGEA).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:38.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vinh Nguyen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-13 01:14:36	ID requested
1	2020-08-31 04:55:50	announced
⏵ 2	2024-10-22 05:11:45	announced	2024-10-22: Updated project metadata.

Nguyen VA, Riddell N, Crewther SG, Faou P, Rajapaksha H, Howells DW, Hankey GJ, Wijeratne T, Ma H, Davis S, Donnan GA, Carey LM, Longitudinal Stroke Recovery Associated With Dysregulation of Complement System-A Proteomics Pathway Analysis. Front Neurol, 11():692(2020) [pubmed]

10.3389/fneur.2020.00692;

submitter keyword: Stroke, human, EDTA plasma, blood, Q exactive HF

Vinh Nguyen
contact affiliation	La Trobe University, School of Psychological Science, Bundoora, Victoria, Australia
contact email	s.crewther@latrobe.edu.au
lab head
Vinh Nguyen
contact affiliation	La Trobe University
contact email	v.nguyen@latrobe.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD015006

PRIDE project URI

• PRIDE
  1. PXD015006
     1. Label: PRIDE project
     2. Name: Stroke Recovery Dominated by Dysregulation of Complement System – A Longitudinal Proteomics Pathway Analysis