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PXD014997

PXD014997 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMS-based proteomic and phosphoproteomic characterization of the AML proteome
DescriptionAcute myeloid leukemia (AML) is a hematological cancer that affects mainly the elderly. Although complete remission (CR) is achieved for the majority of the patients after induction and consolidation therapies, nearly two-thirds relapse within a short interval. Therefore, understanding of the biological factors that determine relapse has become a major interest in clinical AML. In order to identify the proteins and their phosphorylated modifications involved in AML relapse, we performed a global proteome and phosphoproteome study by liquid chromatography tandem mass spectrometry (LC-MS/MS) with primary cells from 41 AML patients at time of diagnosis that were defined as RELAPSE or REL_FREE according to their relapse status after a 5-year clinical follow-up post diagnosis. Our findings showed that the diagnostic sample of patients relapsing had increased levels of RNA processing and decreased expression of V-ATPases proteins along with CDKs and CSK2 activities. LC-MS/MS-based results were further validated with cell proliferation assays using V-ATPase inhibitor bafilomycin A1, CSK2 inhibitor CX-4945, CDK4/6 inhibitor abemaciclib and CDK2/7/9 inhibitor SNS-032. Our study presents molecules that could predict AML relapse and direct new therapeutic strategies that might circumvent more aggressive AML episodes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:01:59.719.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterElise Aasebo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-12 07:28:56ID requested
12020-04-13 23:04:07announced
22020-06-30 04:26:52announced2020-06-30: Updated publication reference for PubMed record(s): 32192169.
32024-10-22 05:02:07announced2024-10-22: Updated project metadata.
Publication List
10.3390/cancers12030709;
Aaseb, ø E, Berven FS, Bartaula-Brevik S, Stokowy T, Hovland R, Vaudel M, D, ø, skeland SO, McCormack E, Batth TS, Olsen JV, Bruserud Ø, Selheim F, Hernandez-Valladares M, Proteome and Phosphoproteome Changes Associated with Prognosis in Acute Myeloid Leukemia. Cancers (Basel), 12(3):(2020) [pubmed]
Keyword List
submitter keyword: LC-MSMS, proteomics, super-SILAC, phosphoproteomics,Acute myeloid leukemia, human AML primary cells
Contact List
Frode Steingrimsen Berven
contact affiliationThe Proteomics Facility of the University of Bergen (PROBE), The Department of Biomedicine, University of Bergen, Bergen, Norway
contact emailfrode.berven@uib.no
lab head
Elise Aasebo
contact affiliationPROBE
contact emailelise.aasebo@uib.no
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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