PXD014990

PXD014990 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry proteomics reveals proteins that function in mTOR regulated selective autophagy
Description	We used proteomic profiling to identify proteins in the mTOR-autophagy axis. Mouse cell lines lacking individual autophagy genes (Atg5 or Ulk1/Ulk2) were treated with an mTOR inhibitor to induce autophagy and cultured in media with either glucose or galactose. Mass spectrometry proteome profiling revealed an elevation of both known autophagy proteins and candidates for new autophagy components.
HostingRepository	PRIDE
AnnounceDate	2020-01-23
AnnouncementXML	Submission_2020-02-17_03:21:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evgenia Shishkova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-12 02:23:06	ID requested
1	2020-01-23 00:39:07	announced
⏵ 2	2020-02-17 03:22:00	announced	2020-02-17: Updated publication reference for PubMed record(s): 31971854.

Stefely JA, Zhang Y, Freiberger EC, Kwiecien NW, Thomas HE, Davis AM, Lowry ND, Vincent CE, Shishkova E, Clark NA, Medvedovic M, Coon JJ, Pagliarini DJ, Mercer CA, Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Autophagy, 16(12):2219-2237(2020) [pubmed]

submitter keyword: Selective autophagy

mTOR

mass spectrometry proteomics

CALCOCO1

LC3

ER-phagy

ATG5

ULK1

ULK2

Joshua J Coon
contact affiliation	Professor, Biomolecular Chemistry and Chemistry at University of Wisconsin - Madison
contact email	jcoon@chem.wisc.edu
lab head
Evgenia Shishkova
contact affiliation	University of Wisconsin - Madison
contact email	shishkova@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014990

PRIDE project URI

• PRIDE
  1. PXD014990
     1. Label: PRIDE project
     2. Name: Mass spectrometry proteomics reveals proteins that function in mTOR regulated selective autophagy