PXD014961

PXD014961 is an original dataset announced via ProteomeXchange.

Title	Network of interactions between ZIKA virus non-structural proteins and human host proteins
Description	The Zika virus (ZIKV) is a mosquito-borne Flavivirus and can be transmitted through an infected mosquito bite or through human-to-human interaction by sexual activity, blood transfusion, breastfeeding or perinatal exposure. After the 2015-2016 outbreak in Brazil, a strong link between ZIKV infection and microcephaly emerged. ZIKV specifically targets human neural progenitor cells, suggesting that proteins encoded by ZIKV bind and inactivate host cell proteins leading to microcephaly. Here, we present a systematic annotation of interactions between human proteins and the seven non-structural ZIKV proteins corresponding to a Brazilian isolate. The interaction network was generated by combining tandem-affinity purification followed by mass spectrometry with yeast two-hybrid screens. We identified 150 human proteins, involved in distinct biological processes, as interactors to ZIKV non-structural proteins. Our interacting network is composed of proteins that have been previously associated with microcephaly in human genetic disorders and/or animal models. This study builds on previously published interacting networks of ZIKV and genes related to autosomal recessive primary microcephaly to generate a catalog of human cellular targets of ZIKV proteins implicated in processes related to microcephaly in humans. Collectively, this data can be used as a resource for future characterization of ZIKV infection biology and help create a basis for the discovery of drugs which may disrupt the interaction and reduce the health damage to the fetus.
HostingRepository	PRIDE
AnnounceDate	2020-06-18
AnnouncementXML	Submission_2020-06-17_22:02:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014961
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Koomen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-08 08:35:06	ID requested
⏵ 1	2020-06-17 22:02:24	announced

Golubeva VA, Nepomuceno TC, Gregoriis G, Mesquita RD, Li X, Dash S, Garcez PP, Suarez-Kurtz G, Izumi V, Koomen J, Carvalho MA, Monteiro ANA, Network of Interactions between ZIKA Virus Non-Structural Proteins and Human Host Proteins. Cells, 9(1):(2020) [pubmed]

submitter keyword: ZIKV, protein-protein interaction, non-structural viral proteins, network

Alvaro Monteiro, PhD
contact affiliation	Moffitt Cancer Center Tampa, FL, USA
contact email	alvaro.monteiro@moffitt.org
lab head
John Koomen
contact affiliation	Moffitt Cancer Center
contact email	john.koomen@moffitt.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014961
     1. Label: PRIDE project
     2. Name: Network of interactions between ZIKA virus non-structural proteins and human host proteins