PXD014959

PXD014959 is an original dataset announced via ProteomeXchange.

Title	YBEY is an essential biogenesis factor for mitochondrial ribosomes
Description	Ribosome biogenesis is a key cellular process involving numerous protein factors, some of which are deeply conserved. This is namely the case of the bacterial endoribonuclease YbeY, whose loss is associated with ribosomal abnormalities, profound changes in gene expression, altered metabolism and death. Surprisingly, YbeY is also widespread among eukaryotes. Here we provide an in-depth characterisation of the human YBEY homologue localising in mitochondria. We show that YBEY loss results in a severe mitochondrial phenotype and complete inability of mitochondria to translate proteins and, consequently, perform oxidative phosphorylation. Through interactions with the important disease protein p32 and other factors, YBEY functions to recruit the mitoribosomal protein uS11m to the nascent small subunit in order to complete the assembly of initiation-competent ribosomal particles. This likely conserved mechanism shows striking similarities with final stages of the cytosolic ribosome biogenesis and may provide an important late checkpoint before the mitoribosome engages in translation.
HostingRepository	PRIDE
AnnounceDate	2021-04-29
AnnouncementXML	Submission_2021-04-29_08:10:41.719.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-08 07:37:14	ID requested
⏵ 1	2021-04-29 08:10:42	announced

Dataset with its publication pending

submitter keyword: YBEY, ribosome, ribosomal protein, translation, mitochondria, oxidative phosphorylation, uS11m, p32

Alexander Smirnov
contact affiliation	UMR7156 - GMGM, Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, CNRS.
contact email	alexandresmirnov@unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014959
     1. Label: PRIDE project
     2. Name: YBEY is an essential biogenesis factor for mitochondrial ribosomes