PXD014957

PXD014957 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry approach to identify mitochondrial poly(A) RNA interacting proteins
Description	To identify mitochondrial RNA-interacting proteins, we developed three related mass spectrometry based methods. In the first method, called mitochondrial crosslinking (MXL), mitochondria of 4-thiouridine labelled cells were isolated, exposed to UV-light and poly(A) RNA was isolated. In the second method, called whole cell crosslinking (WCXL), 4-thiouridine labelled cells were first exposed to UV-light, subsequently mitochondria were isolated followed by a poly(A) RNA extraction. The third method, called ethidium bromide whole cell crosslinking (WCXL_EtBr), is exactly the same as the WCXL method, but the cells are cultured with ethidium bromide to reduce the amount of mitochondrial RNA. In all methods poly(A) RNA was degraded and proteins in the sample were digested and identified using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2019-10-15
AnnouncementXML	Submission_2019-12-11_00:50:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Selma van Esveld
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-08 06:24:24	ID requested
1	2019-10-15 00:55:20	announced
⏵ 2	2019-12-11 00:50:30	announced	2019-12-11: Updated publication reference for PubMed record(s): 31803741.

van Esveld SL, Cans, ı, z-Arda Ş, Hensen F, van der Lee R, Huynen MA, Spelbrink JN, A Combined Mass Spectrometry and Data Integration Approach to Predict the Mitochondrial Poly(A) RNA Interacting Proteome. Front Cell Dev Biol, 7():283(2019) [pubmed]

submitter keyword: mitochondrial RNA, 4-thiouridine labelling & crosslinking, mass spectrometry

Johannes N. Spelbrink
contact affiliation	Radboud Center for Mitochondrial Medicine, Department of Paediatrics, Radboudumc, Nijmegen, The Netherlands
contact email	Hans.Spelbrink@radboudumc.nl
lab head
Selma van Esveld
contact affiliation	RCMM, CMBI, RIMLS, Radboudumc Nijmegen
contact email	selma.vanesveld@radboudumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014957

PRIDE project URI

• PRIDE
  1. PXD014957
     1. Label: PRIDE project
     2. Name: Mass spectrometry approach to identify mitochondrial poly(A) RNA interacting proteins