PXD014955

PXD014955 is an original dataset announced via ProteomeXchange.

Title	SopB and SifA modify the proteome of the Salmonella-containing vacuole in the social amoeba Dictyostelium discoideum
Description	Salmonella enterica serovar Typhimurium is a rod-shaped Gram-negative bacterium. It is a leading cause of gastroenteritis and public health problem. In the environment, it interacts with protozoa, particular amoeba, however the interactions between Salmonella and these eukaryotic microbes have not been addressed in detail. We and others recently described that S. Typhimurium is able to survive in the model amoeba Dictyostelium discoideum requiring the Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2, respectively)-encoded type three secretion systems (T3SSs). In this work, we investigated the role of two particular effector proteins, SopB and SifA that are secreted by either one of the T3SSs. SopB and SifA are involved in the remodelling of the intracellular compartment that contains Salmonella (Salmonella-containing vacuole, SCV) in other cellular models. We combined genetic and proteomics analysis to investigate the roles of SopB and SifA during infections of D. discoideum. We identified over 1,000 proteins per sample performing proteomics on fractions enriched in SCVs from amoeba cells infected with wild-type, sopB or sifA S. Typhimurium. Among them, we observed several Rho GTPases, guanine nucleotide exchange factors and motor proteins. Finally, we decided to evaluate if the changes observed in the proteome from the SCV of wild-type and mutants affect the intracellular survival of S. Typhimurium. These finding suggest that Salmonella exploits this route to survive intracellularly, a process that requires SopB and/or SifA effectors. To our knowledge this is the first proteomic description of the Salmonella intracellular compartment in D. discoideum.
HostingRepository	PRIDE
AnnounceDate	2020-09-23
AnnouncementXML	Submission_2020-09-23_06:57:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Camila Valenzuela
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689; scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S; NCBI TaxID: 588858;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-08 06:24:17	ID requested
⏵ 1	2020-09-23 06:57:51	announced

Valenzuela C, Gil M, Urrutia Í M, Sabag A, Enninga J, Santiviago CA, SopB- and SifA-dependent shaping of the Salmonella-containing vacuole proteome in the social amoeba Dictyostelium discoideum. Cell Microbiol, 23(1):e13263(2021) [pubmed]

submitter keyword: Salmonella, Dictyostelium, Salmonella-containing vacuole

Carlos A. Santiviago
contact affiliation	Laboratorio de Microbiología, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
contact email	csantiviago@ciq.uchile.cl
lab head
Camila Valenzuela
contact affiliation	DIHP, Institut Pasteur
contact email	kamo.valenzuela@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD014955

PRIDE project URI

• PRIDE
  1. PXD014955
     1. Label: PRIDE project
     2. Name: SopB and SifA modify the proteome of the Salmonella-containing vacuole in the social amoeba Dictyostelium discoideum