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PXD014954

PXD014954 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAneuploidy tolerance caused by BRG1 loss allows gain of chromosome 18 and improved fitness
DescriptionAneuploidy results in decreased cellular fitness in many different species and model systems. However, aneuploidy is commonly found in cancer cells and often correlates with aggressive growth and poor prognosis, suggesting that the impact of aneuploidy on cellular fitness is context dependent. The BRG1 (SMARCA4) subunit of the SWI/SNF chromatin remodelling complex is a tumour suppressor that is frequently lost in cancer cells. Here, we used a chromosomally stable cell line to test the effect of BRG1 loss on the evolution of aneuploidy. We find that BRG1 deletion leads to an initial loss of fitness in this cell line that improves over time. The improved fitness correlates with a gain of extra copies of chromosome 18. Notably, changes in pathways that are known to promote tolerance to aneuploidy are evident immediately upon loss of BRG1, providing an environment where karyotype changes associated with a fitness advantage can be explored. At least in some genetic backgrounds, therefore, loss of the SWI/SNF complex can contribute to tumourigenesis through tolerance of aneuploidy.
HostingRepositoryPRIDE
AnnounceDate2022-03-23
AnnouncementXMLSubmission_2022-03-23_03:59:21.863.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Wright
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-08 06:23:39ID requested
12022-03-23 03:59:22announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cancer, LC-MS/MS. TMT,
Contact List
Jyoti Choudhary
contact affiliationInstitute of Cancer Research, London
contact emailjyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliationFunctional Proteomics, Institute Cancer Research & Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute
contact emailjw13@sanger.ac.uk
dataset submitter
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Dataset FTP location
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