PXD014949

PXD014949 is an original dataset announced via ProteomeXchange.

Title	Brugia malayi glycophosphatidylinositol-anchored proteome
Description	A proteomics strategy was used to identify putative GPI-APs from adult B. malayi. Three different sample types were prepared for analysis. Firstly, intact adult worms were treated with PI-PLC to enzymatically release and solubilize the protein away from the lipid moiety. A mock-treatment with no PI-PLC was performed as a negative control. The samples derived from treatment of the intact worms have been named “Surface” but it should be noted that the proteins could originate from any exposed surface like the mouth, vagina, or rectum of the worm. Secondly, a membrane fraction of B. malayi adult female worms was prepared by ultracentrifugation of a total lysate in a sucrose buffer to separate membrane proteins from soluble proteins. This membrane fraction was also treated with PI-PLC or mock-treated without PI-PLC as a negative control. Lastly, a GPI-AP enriched sample was prepared by performing a series of organic solvent partitions to extract GPI-APs from a membrane fraction. This sample was not treated with PI-PLC. Proteins in all three samples types were digested with trypsin and the resulting peptides analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-11_18:27:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fana Mersha
SpeciesList	scientific name: Brugia malayi; NCBI TaxID: 6279;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-08 03:37:37	ID requested
⏵ 1	2019-11-11 18:27:32	announced

Mersha FB, Cortes LK, Luck AN, McClung CM, Ruse CI, Taron CH, Foster JM, Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi. PLoS One, 14(9):e0216849(2019) [pubmed]

submitter keyword: Brugia malayi, GPI-AP, glycophosphatidylinositol, membrane, PI-PLC, phosphatidylinositol specific phospholipase C

Jeremy Michael Foster
contact affiliation	Protein Expression and Modification Division Research Department New England Biolabs United States
contact email	foster@neb.com
lab head
Fana Mersha
contact affiliation	New England Biolabs
contact email	mersha@neb.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014949
     1. Label: PRIDE project
     2. Name: Brugia malayi glycophosphatidylinositol-anchored proteome