PXD014934

PXD014934 is an original dataset announced via ProteomeXchange.

Title	Huanglongbing Citrus Sinensis Phospho and Total Proteomics Data
Description	Huanglongbing (HLB or Citrus Greening Disease) is a destructive citrus disease that has impacted the Florida citrus industry as well as global citrus industry for many years. There is yet to be a practical cure for HLB. Insecticide use targeting the psyllid vector and the burning, destruction and removal of all infected plants are the best solutions as of now. However, this is only a temporary and costly fix. The damage caused by HLB itself, the destruction of infected trees, and indirect effects in other economic sectors costs the Florida economy approximately $900 million per year. There are also problems with insecticide use; the high dosage needed the adequately suppress the transmission of the disease would contaminate the environment, rendering this solution unsustainable. Alternative solutions are needed. In the hopes of uncovering an alternative solution, we investigated the protein and phosphoprotein differences between healthy and diseased citrus plants to identify potential biomarkers essential towards fighting HLB within the trees themselves. For our experimental procedure, we grinded 2 grams of fresh leaf weight from the species Citrus sinensis (sweet orange). Phenol extraction was used to extract the proteins from four biological replicates of the control (healthy) leaves and four biological replicates of the treatment (diseased) leaves. Utilizing a Bradford assay and a 1-D gel electrophoresis, we evaluated the quality and quantity of the samples. Equal amounts of protein from the eight samples were digested with trypsin for 16 hours. Afterwards, we split each sample into two groups: one for phospho-enrichment and the other for total analysis. Phosphopeptides were enriched with the NuTip enrichment procedure for analysis of the phosphopeptides. Total peptides were cleaned up using ZipTip solid phase extraction. We ran the phosphopeptides and total peptides on an LC-MS/MS system, and the raw data files were processed with the Proteome Discoverer software using the citrus database downloaded from NCBI.
HostingRepository	MassIVE
AnnounceDate	2021-01-03
AnnouncementXML	Submission_2021-01-03_21:39:05.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qijie Guan
SpeciesList	scientific name: Citrus sinensis; common name: sweet orange; NCBI TaxID: 2711;
ModificationList	Phospho; Oxidation
Instrument	Orbitrap Fusion; Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-07 17:21:30	ID requested
⏵ 1	2021-01-03 21:39:05	announced

no publication

submitter keyword: HLB, phospho proteomics, iTraq, Citrus Sinensis

Sixue Chen
contact affiliation	University of Florida
contact email	schen@ufl.edu
lab head
Daniel Chen
contact affiliation	University of South Florida
contact email	danielchen8831@gmail.com
lab head
Qijie Guan
contact affiliation	Zhejiang University
contact email	guanqj@zju.edu.cn
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084179/