PXD014933

PXD014933 is an original dataset announced via ProteomeXchange.

Title	ZIKV and DENV-2 sfRNA RNA Pull down Hela extract LC-MSMS
Description	Insect-borne flaviviruses produce a 300-500 base long noncoding RNA, termed sfRNA, by stalling the cellular 5’-3’ exoribonuclease XRN1 via structures located in their 3’ untranslated regions. In this study we demonstrate that the production of sfRNAs by Zika virus results in the repression of XRN1 similar to what we have previously shown for other flaviviruses. Using protein-RNA reconstitution and a stringent RNA pulldown assay, we demonstrate that the sfRNA from both dengue type 2 and Zika viruses interact with a common set of 21 RNA binding proteins that influence post-transcriptional processes in the cell, including splicing, RNA stability and translation. We demonstrate that four of these interacting proteins - DDX6 and EDC3 (RNA decay factors), PHAX1 (a regulator of the biogenesis of the splicing machinery) and APOBEC3C (a nucleic acid editing deaminase) are inherently antiviral restriction factors for Zika virus infection. Furthermore, we demonstrate that cellular mRNA decay regulation as well as cellular RNA splicing are compromised during Zika virus infection. Collectively, these data demonstrate the large extent in which Zika virus sfRNAs interact with cellular RNA binding proteins as well as the potential for widespread dysregulation of post-transcriptional control which likely limits the effective response of the cell to viral infection.
HostingRepository	PRIDE
AnnounceDate	2019-10-01
AnnouncementXML	Submission_2019-10-01_01:11:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014933
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeff Wilusz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Carboxymethyl; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-07 01:13:16	ID requested
⏵ 1	2019-10-01 01:11:23	announced

Dataset with its publication pending

submitter keyword: ZIKV, DENV-2, sfRNA, Hela extract, LC-MSMS

Jeffrey Wilusz
contact affiliation	Microbiology, Immunology and Pathology Department; Colorado State University; USA
contact email	Jeffrey.Wilusz@colostate.edu
lab head
Jeff Wilusz
contact affiliation	Colorado State University
contact email	jeffrey.wilusz@colostate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014933

PRIDE project URI

• PRIDE
  1. PXD014933
     1. Label: PRIDE project
     2. Name: ZIKV and DENV-2 sfRNA RNA Pull down Hela extract LC-MSMS