PXD014928 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome-wide identification of in vitro substrates for P. aeruginosa ClpXP |
Description | we treated the cell lysates of ΔclpX mutant with or without P. aeruginosa ClpXP protease and used Label-free quantitative techniques (LFQ) to identify and quantify the proteolytic targets of ClpXP (p.aeruginosa PAO1). As a result, 3338 proteins were identified and 2493 proteins were quantified. The abundance (LFQ intensity) of 44 proteins were decreased (≥1.5-fold, p < 0.05) by the treatment with ClpXP. Moreover, we observed that there are 31 proteins which have valid LFQ intensity values in all the three biological replicates of the control group but not in the experimental group (i.e., ClpXP-treated). These results suggest that exogenously added ClpXP has a profound effect on the abundance of proteins in our in vitro assays and that those 75 proteins may serve as potential substrates for the P. aeruginosa ClpXP. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:02:49.574.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nana Yang |
SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-06 05:27:29 | ID requested | |
1 | 2020-04-29 02:42:07 | announced | |
⏵ 2 | 2024-10-22 05:02:53 | announced | 2024-10-22: Updated project metadata. |
Publication List
Yang N, Cao Q, Hu S, Xu C, Fan K, Chen F, Yang CG, Liang H, Wu M, Bae T, Lan L, Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus. Mol Microbiol, 114(3):423-442(2020) [pubmed] |
10.1111/mmi.14519; |
Keyword List
submitter keyword: proteolytic targets, Label-free Quantification LC-MS/MS, p.aeruginosa PAO1,ClpXP |
Contact List
Lefu Lan |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | llan@simm.ac.cn |
lab head | |
Nana Yang |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | yaodou888@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD014928 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014928
- Label: PRIDE project
- Name: Proteome-wide identification of in vitro substrates for P. aeruginosa ClpXP