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PXD014926

PXD014926 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Pandas complex adapted for piRNA-guided transposon silencing and heterochromatin formation, Panx-IP-MS
DescriptionThe repression of transposons by the Piwi-interacting RNA (piRNA) pathway is essential to protect animal germ cells. In Drosophila ovaries, Panoramix (Panx) enforces transcriptional silencing by binding to the target-engaged Piwi-piRNA complex, although the precise mechanisms by which this occurs remain elusive. Here, we show that Panx functions together with a germline specific paralogue of a nuclear export factor, dNxf2, and its cofactor dNxt1 (p15), as a ternary complex to suppress transposon expression. Similar to Panx, dNxf2 is essential for animal fertility. Structural and functional analyses demonstrate that dNxf2 binds Panx via its UBA domain, which plays an important role in transposon silencing. Unexpectedly, dNxf2 interacts directly with dNxf1 (TAP), a general nuclear export factor. As a result, dNxf2 prevents dNxf1 from binding to the FG repeats of the nuclear pore complex, a process required for proper RNA export. Transient tethering of dNxf2 to nascent transcripts leads to their nuclear retention. Thus, dNxf2 functions in a Pandas (Panoramix dNxf2 dependent TAP/p15 silencing) complex, which counteracts the canonical RNA exporting machinery and restricts transposons to the nuclear peripheries. Our findings may have broader implications for understanding how RNA metabolism regulates epigenetic gene silencing and heterochromatin formation.
HostingRepositoryiProX
AnnounceDate2019-08-06
AnnouncementXMLSubmission_2019-12-09_22:32:15.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYing Huang
SpeciesList scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-06 03:33:15ID requested
12019-08-06 03:43:56announced
22019-12-09 22:32:16announced2019-12-10: Update publication information.
Publication List
Zhao K, Cheng S, Miao N, Xu P, Lu X, Zhang Y, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y, Yu Y, A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation. Nat Cell Biol, 21(10):1261-1272(2019) [pubmed]
Keyword List
submitter keyword: piRNA, transposon, heterochromatin, dNxf2, Panoramix
Contact List
Yang Yu
contact affiliationInstitute of Biophysics, Chinese Academy of Sciences
contact emailyuyang@ibp.ac.cn
lab head
Ying Huang
contact affiliationShanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
contact emailhuangy@sibcb.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI