PXD014918

PXD014918 is an original dataset announced via ProteomeXchange.

Title	ScandiumIII .
Description	Recently, it has been reported that some metal complexes can act as artificial proteases. In particular, the Lewis acid scandium(III) triflate has been shown to catalyze the cleavage of peptide bonds N- and C-terminal to serine and threonine residues. Therefore, we investigated if this compound can also be used for the cleavage of proteins. For this purpose, several single proteins, the 20S immune-proteasome (17 proteins), and the Universal Proteomics Standard UPS1 (48 proteins) were analyzed by MALDI-MS and/or LC-MS. A high cleavage specificity N-terminal to serine and threonine residues was observed.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:30:22.760.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Koehler
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-05 05:11:55	ID requested
⏵ 1	2024-10-07 10:30:32	announced

Koehler CJ, Thiede B, Predominant cleavage of proteins N-terminal to serines and threonines using scandium(III) triflate. J Biol Inorg Chem, 25(1):61-66(2020) [pubmed]

10.1007/s00775-019-01733-7;

submitter keyword: Endoproteinase, protein cleavage, mass spectrometry,Scandium(III) triflate, Lewis acid

Bernd Thiede
contact affiliation	Department of Biosciences
contact email	bernd.thiede@ibv.uio.no
lab head
Christian Koehler
contact affiliation	University of Oslo
contact email	christian.koehler@ibv.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014918

PRIDE project URI

• PRIDE
  1. PXD014918
     1. Label: PRIDE project
     2. Name: ScandiumIII