PXD014918 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ScandiumIII . |
Description | Recently, it has been reported that some metal complexes can act as artificial proteases. In particular, the Lewis acid scandium(III) triflate has been shown to catalyze the cleavage of peptide bonds N- and C-terminal to serine and threonine residues. Therefore, we investigated if this compound can also be used for the cleavage of proteins. For this purpose, several single proteins, the 20S immune-proteasome (17 proteins), and the Universal Proteomics Standard UPS1 (48 proteins) were analyzed by MALDI-MS and/or LC-MS. A high cleavage specificity N-terminal to serine and threonine residues was observed. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_10:30:22.760.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Koehler |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-05 05:11:55 | ID requested | |
⏵ 1 | 2024-10-07 10:30:32 | announced | |
Publication List
Koehler CJ, Thiede B, Predominant cleavage of proteins N-terminal to serines and threonines using scandium(III) triflate. J Biol Inorg Chem, 25(1):61-66(2020) [pubmed] |
10.1007/s00775-019-01733-7; |
Keyword List
submitter keyword: Endoproteinase, protein cleavage, mass spectrometry,Scandium(III) triflate, Lewis acid |
Contact List
Bernd Thiede |
contact affiliation | Department of Biosciences |
contact email | bernd.thiede@ibv.uio.no |
lab head | |
Christian Koehler |
contact affiliation | University of Oslo |
contact email | christian.koehler@ibv.uio.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014918 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014918
- Label: PRIDE project
- Name: ScandiumIII