PXD014901

PXD014901 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphorylated UPF1 immunoprecipitation in human embryonic kidney 293T cells
Description	HEK293T cells were cultured for 3hr in okadaic acid (200 nM; LC Laboratories) and subsequently collected and lysed using Hypotonic Gentle Lysis Buffer with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). Cellular p-UPF1 was immunoprecipitated using the rabbit polyclonal anti-p-UFP1 S1116 antibody (Millipore) in the presence of 1 U/ml of RNase I (Thermo Fisher Scientific Ambion). Immunoprecipitated material was electrophoresed in 12% SDS-polyacrylamide, and silver-stained bands using SilverQuest Staining Kit (Thermo Fisher Scientific Invitrogen) were excised and subjected to in-gel trypsin digestion. Chromatographed peptides were identified at the Massachusetts Institute of Technology Whitehead Institute Proteomics Core Facility (http://massspec.wi.mit.edu/) using an Orbitrap EliteTM Hybrid Ion Trap-Orbitap Mass Spectrometer with Dionex Ultimate 3000 Rapid Separation Liquid Chromatography (Thermo Fisher Scientific) and Scaffold Software (version Scaffold_4.2.1, Proteome Software Inc.).
HostingRepository	jPOST
AnnounceDate	2020-08-03
AnnouncementXML	Submission_2022-09-18_03:27:49.268.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tatsuaki Kurosaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-02 13:18:40	ID requested
1	2020-08-02 08:00:07	announced
2	2021-02-04 17:07:30	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:27:49	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Phosphorylated UPF1, Nonsense-mediated mRNA decay

submitter keyword: Phosphorylated UPF1, Nonsense-mediated mRNA decay

Contact List

Lynne Maquat
lab head
Tatsuaki Kurosaki
contact affiliation	University of Rochester
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000644/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000644/