PXD014899

PXD014899 is an original dataset announced via ProteomeXchange.

Title	Absolute Quantification of Schistosoma mansoni Esophageal Gland Products
Description	Schistosomes are blood-dwelling helminth parasites causing a debilitating disease in the tropics. Major challenges to control the disease persist and vaccines would provide an additional tool, but their development has been problematic. Rhesus macaques can self-cure following schistosome infection, generating antibodies that target schistosome proteins from the tegument, gut and esophagus, the last of which is the least investigated. We developed a dissection technique that permitted comparative proteomics of the schistosome esophagus and gut for detection of secreted antigens. Shotgun proteome analysis of the male schistosomes esophagus identified 13 proteins encoded by microexon genes (MEG), eleven of which were uniquely located in the esophageal glands. Based on this and transcriptome information, a QconCAT was designed for absolute quantification of selected targets. MEGs 12, 4.2, 4.1 and Venom allergen-like protein 7 were the mostly abundant, spanning over 245-6 million copies per cell, while aspartyl protease, palmitoyl thioesterase and a galactosyl transferase were present at <1 million cpc. Antigenic variation by alternative splicing of MEG proteins was confirmed together with a specialised machinery for protein glycosylation in the esophagus. Moreover, some gastrodermal secretions were highly enriched in the gut, while others were more uniformly distributed throughout the parasite, potentially indicating lysosomal activity. Collectively, our findings provide a more rational, better-oriented selection of schistosome vaccine candidates in the context of a proven model of protective immunity.
HostingRepository	PanoramaPublic
AnnounceDate	2019-11-22
AnnouncementXML	Submission_2019-11-22_07:43:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leandro Neves
SpeciesList	scientific name: Schistosoma mansoni; NCBI TaxID: 6183;
ModificationList	Carbamidomethyl; Oxidation; Label:13C(6)
Instrument	Xevo TQ-S

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-02 12:11:30	ID requested
⏵ 1	2019-11-22 07:43:25	announced

Neves LX, Wilson RA, Brownridge P, Harman VM, Holman SW, Beynon RJ, Eyers CE, DeMarco R, Castro-Borges W, Pinpoints Secreted Proteins for Vaccine Development. J Proteome Res, 19(1):314-326(2020) [pubmed]

submitter keyword: Schistosoma mansoni, esophageal gland, gastrodermis, microexon genes, quantitative proteomics, QconCAT

William de Castro Borges
contact affiliation	Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, Minas Gerais, Brasil
contact email	wborges@ufop.edu.br
lab head
Leandro Neves
contact affiliation	Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, Minas Gerais, Brasil
contact email	leandroxneves@gmail.com
dataset submitter

Panorama Public dataset URI