PXD014897

PXD014897 is an original dataset announced via ProteomeXchange.

Title	Salvage pathway maintains functional respiratory complex I
Description	Regulation of the turnover of complex I (CI), the largest mitochondrial respiratory chain complex remains enigmatic, despite huge advancement in understanding its structure and the assembly steps in recent years. Although some studies indicated that CI might be remodelled in response to various stimuli, the mechanism mediating these putative changes is unknown. Here, we report that the NADH-oxidising N-module which sits on the tip of the CI peripheral arm is turned over at a higher rate and largely independently of the rest of the complex. The N-module turnover is mediated by mitochondrial matrix protease ClpXP, which selectively removes and degrades damaged core subunits. The observed mechanism seems to be a safeguard against accumulation of dysfunctional CI arising from inactivation of the N-module subunits due to attrition caused by extensive redox activity. This CI salvage pathway maintains highly functional CI and healthy mitochondria through an energetically favourable mechanism that demands much lower energetic cost than de novo synthesis and reassembly of the entire CI.
HostingRepository	PRIDE
AnnounceDate	2020-03-05
AnnouncementXML	Submission_2020-04-22_03:48:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karolina Szczepanowska
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-02 07:46:23	ID requested
1	2020-03-05 06:15:16	announced
⏵ 2	2020-04-22 03:49:01	announced	2020-04-22: Updated publication reference for PubMed record(s): 32242014.

Szczepanowska K, Senft K, Heidler J, Herholz M, Kukat A, H, ö, hne MN, Hofsetz E, Becker C, Kaspar S, Giese H, Zwicker K, Guerrero-Castillo S, Baumann L, Kauppila J, Rumyantseva A, M, ü, ller S, Frese CK, Brandt U, Riemer J, Wittig I, Trifunovic A, A salvage pathway maintains highly functional respiratory complex I. Nat Commun, 11(1):1643(2020) [pubmed]

submitter keyword: Mitochondrial respiratory complex I

Proteolytic control

Salvage pathway

Respiratory deficiency

ClpXP protease;

Aleksandra Trifunovic
contact affiliation	Institute for Mitochondrial Diseases and Aging, Medical Faculty, University of Cologne, D-50931 Cologne, Germany,
contact email	aleksandra.trifunovic@uk-koeln.de
lab head
Karolina Szczepanowska
contact affiliation	Institute of Mitochondrial Disease and Ageing, Medical Faculty, University of Cologne
contact email	karolina.szczepanowska@uk-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD014897

PRIDE project URI

• PRIDE
  1. PXD014897
     1. Label: PRIDE project
     2. Name: Salvage pathway maintains functional respiratory complex I