PXD014888

PXD014888 is an original dataset announced via ProteomeXchange.

Title	Protein compositions of extracellular vesicles released from Staphylococcus aurues JE2 and its lgt mutant
Description	This project detected the protein composition of extracellular vesicles (EV) produced by S. aureus WT JE2 strain and its lgt mutant (lipoprotein deficient mutant). Our recent study found that mutation of lgt in S. aureus USA300 JE2 strain significantly increased EV production in S. aureus and decreased the pore-forming toxin content of vesicles, indicatiung that lipoproteins modulate the EV biogenesis of S. aureus. To gain a better understanding of this process, we detected the protein compositions associated with EVs purified from both WT and lgt mutant EVs by LC-MS/MS. Compared to WT EVs, more cytosoli proteins and less extracellular proteins were identified in lgt mutant EVs. A total of 180 and 198 proteins were identified from WT and lgt mutant EVs, respectively.In addition 27.2% and 33.8% of identified proteins were unique to WT or lgt mutant EVs respectively.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_13:41:51.052.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaogang Wang
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-02 02:42:23	ID requested
⏵ 1	2021-09-08 13:41:51	announced

Wang X, Koffi PF, English OF, Lee JC, Extracellular Vesicles: A Story of Toxicity and the Stress of 2020. Toxins (Basel), 13(2):(2021) [pubmed]

submitter keyword: vesicle, Staphylococcus aureus, lgt

Jean C. Lee
contact affiliation	Department of Medicine (Infectious Diseases) Brigham and Women's Hospital and Harvard Medical School
contact email	jclee@bwh.harvard.edu
lab head
Xiaogang Wang
contact affiliation	Harvard Medical school
contact email	xwang39@bwh.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD014888

PRIDE project URI

• PRIDE
  1. PXD014888
     1. Label: PRIDE project
     2. Name: Protein compositions of extracellular vesicles released from Staphylococcus aurues JE2 and its lgt mutant