PXD014886

PXD014886 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial reprogramming using dCas9 boosts glia-to-neuron conversion
Description	Astrocyte-to-neuron conversion has developed into a promising avenue for neuronal replacement therapy. Neurons depend critically on mitochondria function and often die by ferroptosis during the conversion process. Here we examined the extent of adequate mitochondrial reprogramming by morphology and proteome analysis. While mitochondria profoundly changed their morphology during Neurogenin2 (Neurog2) – or Achaete-scute homolog 1 (Ascl1)-mediated astrocyte-to-neuron reprogramming, we found neuron-specific mitochondrial proteins, here identified in a comprehensive proteome analysis of isolated mitochondria from primary neurons and astrocytes, to be only partially and at late stages regulated during the process. To improve this, we used dCas9 technology to induce neuron-specific mitochondrial proteins early during reprogramming. This resulted not only in increased conversion efficiency, but also in faster neuronal generation. Taken together, reprogramming mitochondria in a cell type-specific manner has powerful effects on astrocyte-to-neuron conversion, suggesting mitochondria to be a driving force in this process.
HostingRepository	PRIDE
AnnounceDate	2021-03-29
AnnouncementXML	Submission_2021-03-29_01:08:59.779.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliane Merl-Pham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-02 02:41:26	ID requested
⏵ 1	2021-03-29 01:09:00	announced

Dataset with its publication pending

submitter keyword: mitochondria, Cas9, astrocytes, neurons

Magdalena Götz
contact affiliation	Physiological Genomics, Biomedical Center, Ludwig Maximilians Universitaet Munich, Germany; Institute for Stem Cell Research, Helmholtz Center Munich, Neuherberg, Germany
contact email	Magdalena.goetz@helmholtz-muenchen.de
lab head
Juliane Merl-Pham
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD014886

PRIDE project URI

• PRIDE
  1. PXD014886
     1. Label: PRIDE project
     2. Name: Mitochondrial reprogramming using dCas9 boosts glia-to-neuron conversion