PXD014882 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics reveals the protective effects of Huangqi decoction against intrahepatic cholestasis by inhibiting NF-κB/IL-6/STAT3 signaling pathwa |
Description | Intrahepatic cholestasis (IC) is a common symptom of liver diseases but with limited treatment. Huangqi decoction (HQD), a classic herbal medicine, has shown protective effects against IC. In this study, the iTRAQ-based quantitative proteomics was performed to investigate the potential mechanism of HQD on alpha-naphthylisothiocyanate (ANIT) induced IC, resulting in 2,796 quantified proteins across all the samples, including 270 differentially expressed proteins under HQD treatment. Fuzzy c-means (FCM) clustering analysis of these 270 proteins revealed that the pro-inflammatory proteins, such as LCN2, SAA1, FGG, FGA, and FGB, were assigned in the Cluster 1 (up-regulated by ANIT, and down-regulated by HQD). Following functional bioinformatics analysis and protein-protein interaction (PPI) network analysis represented that these pro-inflammatory proteins were involved in the STAT3 signaling pathway. Further real-time PCR and Western blot experiments confirmed that the expression of these proteins was consistent with the proteomic results. Moreover, HQD treatment decreased the phosphorylation of STAT3 that induced by ANIT. And Western blot experiments also revealed that HQD could decrease phosphorylation of NF-κB and down-regulate the expression inflammatory genes IL-6, and therefore inhibit IL-6/STATA3 signaling pathway. In summary, the present study suggested that HQD treatment may ameliorate intrahepatic cholestasis via inhibiting the NF-κB/IL-6/STAT3 signaling pathway. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:58:03.242.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qian Liu |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-01 09:01:30 | ID requested | |
1 | 2020-01-13 00:37:50 | announced | |
⏵ 2 | 2024-10-22 04:58:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.jproteome.9b00563; |
Wu JS, Liu Q, Fang SH, Liu X, Zheng M, Wang TM, Zhang H, Liu P, Zhou H, Ma YM, B/IL-6/STAT3 Signaling Pathway. J Proteome Res, 19(2):677-687(2020) [pubmed] |
Keyword List
submitter keyword: Intrahepatic cholestasis |
Huangqi decoction |
Proteomics |
NF-κB |
IL-6 |
STAT3 |
Contact List
Hu Zhou |
contact affiliation | Department of Analytical Chemistry and CAS Key Laboratory for Receptor Research, Shanghai Institute of Materia Medica |
contact email | zhouhu@simm.ac.cn |
lab head | |
Qian Liu |
contact affiliation | Shanghai Institute of Materia Medica |
contact email | liuqian_scu@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014882
- Label: PRIDE project
- Name: Quantitative proteomics reveals the protective effects of Huangqi decoction against intrahepatic cholestasis by inhibiting NF-κB/IL-6/STAT3 signaling pathwa