PXD014882

PXD014882 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics reveals the protective effects of Huangqi decoction against intrahepatic cholestasis by inhibiting NF-κB/IL-6/STAT3 signaling pathwa
Description	Intrahepatic cholestasis (IC) is a common symptom of liver diseases but with limited treatment. Huangqi decoction (HQD), a classic herbal medicine, has shown protective effects against IC. In this study, the iTRAQ-based quantitative proteomics was performed to investigate the potential mechanism of HQD on alpha-naphthylisothiocyanate (ANIT) induced IC, resulting in 2,796 quantified proteins across all the samples, including 270 differentially expressed proteins under HQD treatment. Fuzzy c-means (FCM) clustering analysis of these 270 proteins revealed that the pro-inflammatory proteins, such as LCN2, SAA1, FGG, FGA, and FGB, were assigned in the Cluster 1 (up-regulated by ANIT, and down-regulated by HQD). Following functional bioinformatics analysis and protein-protein interaction (PPI) network analysis represented that these pro-inflammatory proteins were involved in the STAT3 signaling pathway. Further real-time PCR and Western blot experiments confirmed that the expression of these proteins was consistent with the proteomic results. Moreover, HQD treatment decreased the phosphorylation of STAT3 that induced by ANIT. And Western blot experiments also revealed that HQD could decrease phosphorylation of NF-κB and down-regulate the expression inflammatory genes IL-6, and therefore inhibit IL-6/STATA3 signaling pathway. In summary, the present study suggested that HQD treatment may ameliorate intrahepatic cholestasis via inhibiting the NF-κB/IL-6/STAT3 signaling pathway.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:03.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-01 09:01:30	ID requested
1	2020-01-13 00:37:50	announced
⏵ 2	2024-10-22 04:58:04	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00563;

Wu JS, Liu Q, Fang SH, Liu X, Zheng M, Wang TM, Zhang H, Liu P, Zhou H, Ma YM, B/IL-6/STAT3 Signaling Pathway. J Proteome Res, 19(2):677-687(2020) [pubmed]

submitter keyword: Intrahepatic cholestasis

Huangqi decoction

Proteomics

NF-κB

IL-6

STAT3

Hu Zhou
contact affiliation	Department of Analytical Chemistry and CAS Key Laboratory for Receptor Research, Shanghai Institute of Materia Medica
contact email	zhouhu@simm.ac.cn
lab head
Qian Liu
contact affiliation	Shanghai Institute of Materia Medica
contact email	liuqian_scu@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014882
     1. Label: PRIDE project
     2. Name: Quantitative proteomics reveals the protective effects of Huangqi decoction against intrahepatic cholestasis by inhibiting NF-κB/IL-6/STAT3 signaling pathwa