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PXD014882

PXD014882 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics reveals the protective effects of Huangqi decoction against intrahepatic cholestasis by inhibiting NF-κB/IL-6/STAT3 signaling pathwa
DescriptionIntrahepatic cholestasis (IC) is a common symptom of liver diseases but with limited treatment. Huangqi decoction (HQD), a classic herbal medicine, has shown protective effects against IC. In this study, the iTRAQ-based quantitative proteomics was performed to investigate the potential mechanism of HQD on alpha-naphthylisothiocyanate (ANIT) induced IC, resulting in 2,796 quantified proteins across all the samples, including 270 differentially expressed proteins under HQD treatment. Fuzzy c-means (FCM) clustering analysis of these 270 proteins revealed that the pro-inflammatory proteins, such as LCN2, SAA1, FGG, FGA, and FGB, were assigned in the Cluster 1 (up-regulated by ANIT, and down-regulated by HQD). Following functional bioinformatics analysis and protein-protein interaction (PPI) network analysis represented that these pro-inflammatory proteins were involved in the STAT3 signaling pathway. Further real-time PCR and Western blot experiments confirmed that the expression of these proteins was consistent with the proteomic results. Moreover, HQD treatment decreased the phosphorylation of STAT3 that induced by ANIT. And Western blot experiments also revealed that HQD could decrease phosphorylation of NF-κB and down-regulate the expression inflammatory genes IL-6, and therefore inhibit IL-6/STATA3 signaling pathway. In summary, the present study suggested that HQD treatment may ameliorate intrahepatic cholestasis via inhibiting the NF-κB/IL-6/STAT3 signaling pathway.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:58:03.242.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQian Liu
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-01 09:01:30ID requested
12020-01-13 00:37:50announced
22024-10-22 04:58:04announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.9b00563;
Wu JS, Liu Q, Fang SH, Liu X, Zheng M, Wang TM, Zhang H, Liu P, Zhou H, Ma YM, B/IL-6/STAT3 Signaling Pathway. J Proteome Res, 19(2):677-687(2020) [pubmed]
Keyword List
submitter keyword: Intrahepatic cholestasis
Huangqi decoction
Proteomics
NF-κB
IL-6
STAT3
Contact List
Hu Zhou
contact affiliationDepartment of Analytical Chemistry and CAS Key Laboratory for Receptor Research, Shanghai Institute of Materia Medica
contact emailzhouhu@simm.ac.cn
lab head
Qian Liu
contact affiliationShanghai Institute of Materia Medica
contact emailliuqian_scu@163.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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