PXD014880

PXD014880 is an original dataset announced via ProteomeXchange.

Title	Effect of leucine and pyruvate on the proteomic profile of cysteine treated wt and ncl1 deleted strain
Description	Cell requires optimal amount of nutrients for the synthesis of macromolecules like lipid, protein and nucleotides. There is a very limited knowledge about how cells maintain homeostasis in excess nutrients. By using Yeast as a model system we have shown that some aminoacids are toxic at higher concentration. Using cysteine as a reference molecule we delineated different biological pathways/processes required for survival. Leucine and pyruvate are two molecules which can rescue cysteine induced toxicity.Through a comprehensive genetic screen we show that leucine mediated effect depends on a tRNA methyltransferase (Ncl1), absence of which decouples transcription and translation in the cell. Using proteomics approach we have determined the altered pathway in both wild type and ncl1 deletion strain and the effect of leucine and pyruvate on the proteome of cysteine treated cells.
HostingRepository	PRIDE
AnnounceDate	2019-08-13
AnnouncementXML	Submission_2019-08-13_03:38:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rahul Chakraborty
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-01 08:01:37	ID requested
⏵ 1	2019-08-13 03:38:13	announced

Dataset with its publication pending

submitter keyword: Cysteine,Yeast,t-RNA methyl transferase

Shantanu Sengupta
contact affiliation	CSIR-Institute of Genomics and Integrative Biology
contact email	shantanus@igib.res.in
lab head
Rahul Chakraborty
contact affiliation	CSIR-IGIB
contact email	rahul.c@igib.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014880
     1. Label: PRIDE project
     2. Name: Effect of leucine and pyruvate on the proteomic profile of cysteine treated wt and ncl1 deleted strain