PXD014872

PXD014872 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni
Description	Schistosomes are blood-dwelling helminth parasites causing a debilitating disease in the tropics. Major challenges to control persist and vaccines would provide an additional tool, but their development has been problematic. During the self-cure response of Rhesus macaques, antibodies target proteins from the tegument, gut and esophagus, the last of which is the least investigated. We developed a dissection technique that permitted comparative proteomics on the schistosome esophagus and gut. A shotgun analysis applied to male heads identified 13 MEG proteins, eleven of which were uniquely located in the esophageal glands. Antigenic variation by alternative splicing of MEG proteoforms was confirmed together with a specialised machinery for protein glycosylation in the esophagus. Moreover some gastrodermal secretions were highly enriched in the gut, while others were more uniformly distributed, potentially as markers of lysosomal activity. Collectively, our findings provide a more rational, better-oriented selection of schistosome vaccine candidates in the context of a proven model of protective immunity.
HostingRepository	PRIDE
AnnounceDate	2019-12-11
AnnouncementXML	Submission_2019-12-11_05:30:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014872
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leandro Neves
SpeciesList	scientific name: Schistosoma mansoni (Blood fluke); NCBI TaxID: 6183;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive HF; Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-01 03:29:22	ID requested
1	2019-12-11 02:21:48	announced
⏵ 2	2019-12-11 05:30:12	announced	2019-12-11: Updated project metadata.

Neves LX, Wilson RA, Brownridge P, Harman VM, Holman SW, Beynon RJ, Eyers CE, DeMarco R, Castro-Borges W, Pinpoints Secreted Proteins for Vaccine Development. J Proteome Res, 19(1):314-326(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Quantitative proteomics, schistosoma mansoni, esophageal gland, microexon genes, gastrodermis

William de Castro Borges
contact affiliation	Departamento de Ciências Biológicas, Laboratório de Enzimologia e Proteômica, Universidade Federal de Ouro Preto, Brazil
contact email	wborges@ufop.edu.br
lab head
Leandro Neves
contact affiliation	Postdoctoral research assistant at Brazilian National Biosciences Laboratory (LNBio), CNPEM, Campinas, Brazil
contact email	leandroxneves@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014872

PRIDE project URI

• PRIDE
  1. PXD014872
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni