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PXD014871

PXD014871 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic changes in human sperm during sequential in vitro capacitation and acrosome reaction.
DescriptionThe male gamete is not completely mature after ejaculation and requires further events in the female genital tract to acquire fertilizing ability, including the processes of capacitation and acrosome reaction. In order to shed light on dynamic protein changes experienced by the sperm cell in preparation for fertilization, a comprehensive quantitative proteomic profiling based on isotopic peptide labeling and liquid chromatography followed by tandem mass spectrometry was performed on spermatozoa from three donors of proven fertility under three sequential conditions: purification with density gradient centrifugation, incubation with capacitation medium, and induction of acrosome reaction by the exposure to the calcium ionophore A23187. After applying strict selection criteria for peptide quantification and for statistical analyses, 36 proteins with significant changes in their relative abundance within sperm protein extracts were detected. Moreover, the presence of peptide residues potentially harboring sites for post-translational modification was revealed, suggesting that protein modification may be an important mechanism in sperm maturation. In this regard, increased levels of proteins mainly involved in motility and signaling, both regulated by protein modifiers, were detected in sperm lysates following incubation with capacitation medium. In contrast, less abundant proteins in acrosome-reacted cell lysates did not contain potentially modifiable residues, suggesting the possibility that all those proteins might be relocated or released during the process. Protein-protein interaction analysis revealed a subset of proteins potentially involved in sperm maturation, including ERLIN2, GGH, TMED 10, and ATP synthases. These results contribute to the current knowledge of the molecular basis of human fertilization and propose new candidates to be validated as modulators of male fertility.
HostingRepositoryPRIDE
AnnounceDate2019-12-18
AnnouncementXMLSubmission_2020-01-14_07:23:25.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD014871
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJudit Castillo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListresidues isobaric at 113.084064 Da; TMT6plex; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-01 03:29:17ID requested
12019-11-28 02:19:14announced
22019-12-18 00:30:29announced2019-12-18: Updated project metadata.
32020-01-14 07:23:27announced2019-12-18: Updated project metadata.
2020-01-14: Updated publication reference for PubMed record(s): 31824947.
Publication List
Castillo J, Bogle OA, Jodar M, Torabi F, Delgado-Dueñas D, Estanyol JM, Ballescà JL, Miller D, Oliva R, Capacitation and Acrosome Reaction. Front Cell Dev Biol, 7():295(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: sperm, spermatozoa, capacitation, acrosome reaction, proteomics, mass spectrometry, TMT labeling
Contact List
Rafael Oliva Virgili
contact affiliationMolecular Biology of Reproduction and Development Research Group; Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS); Fundació Clínic per a la Recerca Biomèdica (FCRB); Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universitat de Barcelona; Biochemistry and Molecular Genetics Service, Hospital Clínic, Barcelona, Spain
contact emailroliva@ub.edu
lab head
Judit Castillo
contact affiliationInstitut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona
contact emailjuditcastillo@ub.edu
dataset submitter
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Dataset FTP location
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