PXD014849

PXD014849 is an original dataset announced via ProteomeXchange.

Title	Obesity significantly alters the human sperm proteome, with implications for fertility
Description	While obesity has links to poor semen parameters and reduced fertility, the causative links between obesity and male infertility are unclear, particularly on a molecular level. We investigated how obesity impacts the human sperm proteome, to further understand any implications for fertility. Sperm protein lysates from 5 healthy weight (BMI < 25) or obese (BMI>30) men were FASP digested, analysed by LC-MS/MS and quantitatively compared by label free quantification. Findings were confirmed for representative proteins by immunofluorescence staining. 2034 proteins were confidently identified, with 24 significantly less abundant (fold change <0.5) and 3 significantly more abundant (fold change >1.5)in sperm of obese men. These proteins were involved in a range of biological processes, including oxidative stress (GSS, NDUFS2, JAGN1, USP14, ADH5), inflammation (SUGT1, LTA4H), translation (EIF3F, EIF4A2, CSNK1G1), DNA damage repair (UBEA4) and sperm function (NAPA, RNPEP, BANF2). These results suggest that oxidative stress and inflammation are closely tied to reproductive dysfunction in obese men. These processes likely impact protein translation and folding during spermatogenesis, leading to poor sperm function and subfertility. The observation of these changes in normozoospermic men further suggests that traditional clinical semen assessment fails to detect important biological changes in spermatozoa which may compromise fertility.
HostingRepository	PRIDE
AnnounceDate	2020-02-18
AnnouncementXML	Submission_2020-02-18_02:36:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taylor Pini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-31 01:24:58	ID requested
⏵ 1	2020-02-18 02:36:52	announced

Pini T, Parks J, Russ J, Dzieciatkowska M, Hansen KC, Schoolcraft WB, Katz-Jaffe M, Obesity significantly alters the human sperm proteome, with potential implications for fertility. J Assist Reprod Genet, 37(4):777-787(2020) [pubmed]

submitter keyword: Human, sperm, obesity, LC-MS/MS, LFQ

Taylor Pini
contact affiliation	Colorado Center for Reproductive Medicine, CO, USA
contact email	tpini@flcolo.com
lab head
Taylor Pini
contact affiliation	Colorado Center for Reproductive Medicine
contact email	tpini@flcolo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014849
     1. Label: PRIDE project
     2. Name: Obesity significantly alters the human sperm proteome, with implications for fertility