PXD014845

PXD014845 is an original dataset announced via ProteomeXchange.

Title	Global analysis of the human cytomegalovirus interactome identifies degradation hubs, domain associations and viral protein functions
Description	Human cytomegalovirus (HCMV) is an important pathogen that extensively modulates host cells, downregulating >900 human proteins over the course of viral replication and degrading ≥133 proteins shortly after infection. The mechanism of degradation of most host proteins remains unresolved, and the functions of many viral proteins are incompletely characterised. We performed a systematic interactome analysis of 169 canonical HCMV proteins, and a subset of non-canonical HCMV proteins, in infected cells. This identified an extensive network of >3,400 virus-host and >150 virus-virus protein interactions, providing insights into novel functions for multiple viral genes. Domain analysis predicted binding of the viral UL25 protein to the SH3 domains of NCK Adaptor Protein 1. Viral interacting proteins were identified for 31/133 degraded host targets. Finally, the uncharacterised, non-canonical ORFL147C protein was found to interact with elements of the mRNA splicing machinery, and a mutational study suggested its importance in viral replication. The interactome data will be important for future studies of herpesvirus infection.
HostingRepository	PRIDE
AnnounceDate	2020-01-22
AnnouncementXML	Submission_2020-01-22_05:40:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Weekes
SpeciesList	scientific name: Human herpesvirus 5 strain Merlin; NCBI TaxID: 295027; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-31 01:24:03	ID requested
⏵ 1	2020-01-22 05:40:01	announced

Nobre LV, Nightingale K, Ravenhill BJ, Antrobus R, Soday L, Nichols J, Davies JA, Seirafian S, Wang EC, Davison AJ, Wilkinson GW, Stanton RJ, Huttlin EL, Weekes MP, Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. Elife, 8():(2019) [pubmed]

submitter keyword: proteomics

systems virology

human cytomegalovirus

host-pathogen interaction

immune evasion

protein-protein interaction

Michael Weekes
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, CB2 0XY, UK
contact email	mpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliation	University of Cambridge
contact email	mpw1001@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014845
     1. Label: PRIDE project
     2. Name: Global analysis of the human cytomegalovirus interactome identifies degradation hubs, domain associations and viral protein functions