PXD014832

PXD014832 is an original dataset announced via ProteomeXchange.

Title	S6K1 Phosphoproteome in Tsc1-null mouse kidney iMCD3 cells
Description	Loss of Tsc1 in the kidneys gives rise to rapid polycystogenesis and kidney failure due to the hyperactivation of mTOR complex I (mTORC1). One of the major downstream targets of mTORC1 is S6K1, which is responsible for driving many of the effector functions of mTOR. Strikingly, the loss of S6k1 in the context of hyperactivated mTOR is sufficient to reverse the generation of polycystic kidneys. In an effort to uncover novel direct S6k1 substrates involved in the generation of cystic kidneys, we generated Tsc1-null and Tsc1/S6k1-double null murine inner medullary collecting duct (iMCD3) cell lines using CRISPR/Cas9. Using these two cell lines, we conducted a phosphoproteome screen to search for novel candidates. Using a combination of antibody-motif enrichment and metal affinity enrichment, we provide a comprehensive phosphoproteome profile between these two genotypes.
HostingRepository	PRIDE
AnnounceDate	2020-08-11
AnnouncementXML	Submission_2020-08-11_04:34:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Kuperwasser
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-30 06:21:48	ID requested
⏵ 1	2020-08-11 04:34:12	announced

Bonucci M, Kuperwasser N, Barbe S, Koka V, de Villeneuve D, Zhang C, Srivastava N, Jia X, Stokes MP, Bienaim, é F, Verkarre V, Lopez JB, Jaulin F, Pontoglio M, Terzi F, Delaval B, Piel M, Pende M, mTOR and S6K1 drive polycystic kidney by the control of Afadin-dependent oriented cell division. Nat Commun, 11(1):3200(2020) [pubmed]

ProteomeXchange project tag: Kidney Urine (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Kidney, S6k1, Tsc1, mTOR, Phosphoproteome

Mario Pende
contact affiliation	Laboratory of Cell Growth Control by Nutrients, Inserm U1151, Necker Enfants Malades Institute (INEM), Faculté de Médecine Paris Descartes, Leriche Building 14 Rue Maria Helena Vieira da Silva Paris 75014 France
contact email	mario.pende@inserm.fr
lab head
Nicolas Kuperwasser
contact affiliation	INSERM
contact email	nicolas.kuperwasser@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD014832

PRIDE project URI

• PRIDE
  1. PXD014832
     1. Label: PRIDE project
     2. Name: S6K1 Phosphoproteome in Tsc1-null mouse kidney iMCD3 cells