PXD014829

PXD014829 is an original dataset announced via ProteomeXchange.

Title	Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites
Description	The centrosome is the master orchestrator of mitotic spindle formation and chromosome segregation in animal cells. Centrosome abnormalities are frequently observed in cancer, but little is known of their origin and about pathways affecting centrosome homeostasis. Here we show that autophagy preserves centrosome organization and stability through selective turnover of centriolar satellite components, a process we termed doryphagy. Autophagy targets the satellite organizer PCM1 by interacting with GABARAPs via a C-terminal LIR motif. Accordingly, autophagy deficiency results in accumulation of large abnormal centriolar satellites and a resultant dysregulation of centrosome composition. These alterations have critical impact on centrosome stability and lead to mitotic centrosome fragmentation and unbalanced chromosome segregation. Our findings identify doryphagy as an important centrosome-regulating pathway and bring mechanistic insights to the link between autophagy dysfunction and chromosomal instability. In addition, we highlight the vital role of centriolar satellites in maintaining centrosome integrity.
HostingRepository	PRIDE
AnnounceDate	2019-09-12
AnnouncementXML	Submission_2019-09-18_08:16:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Lubas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-30 05:52:06	ID requested
1	2019-09-12 07:17:03	announced
⏵ 2	2019-09-18 08:16:10	announced	2019-09-18: Updated publication reference for PubMed record(s): 31519908.

Holdgaard SG, Cianfanelli V, Pupo E, Lambrughi M, Lubas M, Nielsen JC, Eibes S, Maiani E, Harder LM, Wesch N, Foged MM, Maeda K, Nazio F, de la Ballina LR, D, ö, tsch V, Brech A, Frankel LB, J, ä, ä, ttel, ä M, Locatelli F, Barisic M, Andersen JS, Bekker-Jensen S, Lund AH, Rogov VV, Papaleo E, Lanzetti L, De Zio D, Cecconi F, Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun, 10(1):4176(2019) [pubmed]

submitter keyword: Autophagy, centriolar satellites, centrosome, centrosome fragmentation, CEP131, GABARAP, mitosis, PCM1, selective autophagy

Anders Lund
contact affiliation	Anders H. Lund Professor, PhD Biotech Research and Innovation Centre University of Copenhagen Ole Maaloes Vej 5 DK-2200 N Copenhagen Denmark
contact email	anders.lund@bric.ku.dk
lab head
Michal Lubas
contact affiliation	University of Southern Denmark
contact email	mlubas@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014829

PRIDE project URI

• PRIDE
  1. PXD014829
     1. Label: PRIDE project
     2. Name: Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites