PXD014825

PXD014825 is an original dataset announced via ProteomeXchange.

Title	Identification of cellular CDK9 substrates using an analog-sensitive cell line
Description	Analog-sensitive kinase technology allows for specific inhibition of a given kinase. Here we utilize an analog-sensitive CDK9 cell line to determine the impact of CDK9 inhibition on the phospho-proteome of human cells. Specifically, we aim to identify substrates of CDK9 in a quantitative way using stable isotope labeling by amino acids in cell culture (SILAC) followed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2019-11-11
AnnouncementXML	Submission_2020-01-02_07:54:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-30 05:44:18	ID requested
1	2019-11-11 02:39:16	announced
⏵ 2	2020-01-02 07:54:42	announced	2020-01-02: Updated publication reference for PubMed record(s): 31857848.

Decker TM, Forn, é I, Straub T, Elsaman H, Ma G, Shah N, Imhof A, Eick D, Analog-sensitive cell line identifies cellular substrates of CDK9. Oncotarget, 10(65):6934-6943(2019) [pubmed]

submitter keyword: Protein phosphorylation, P-TEFb, Analog-sensitive, mass spectrometry

Prof. Dr. Dirk Eick
contact affiliation	Research Unit Molecular Epigenetics German Research Center for Environmental Health (GmbH) Helmholtz Zentrum M?nchen Marchioninistra?e 25 81377 Munich Tel. +49 89 3187-1512 Fax +49 89 3187-1200
contact email	eick@helmholtz-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014825

PRIDE project URI

• PRIDE
  1. PXD014825
     1. Label: PRIDE project
     2. Name: Identification of cellular CDK9 substrates using an analog-sensitive cell line