PXD014807

PXD014807 is an original dataset announced via ProteomeXchange.

Title	Domain model explains propagation dynamics and stability of K27 and K36 methylation landscape
Description	Chromatin states must be stably maintained during cell proliferation to uphold cellular identity and genome integrity. Inheritance of histone modifications across cell division is thought to be central in this process. However, the histone modification landscape is challenged by the incorporation of new unmodified histones during each cell cycle and the principles that govern heritability remain poorly defined. Here, we take a quantitative approach and develop a reusable computational model that describes propagation of K27 and K36 methylation states. We measure combinatorial K27 and K36 methylation patterns by quantitative mass spectrometry on subsequent generations of histones in the presence and absence of enzymatic inhibition. Our modelling rejects active global demethylation and invoke the existence of 8 domains defined by distinct methylation endpoints. We find that K27me3 on pre- existing histones stimulates the rate of de novo K27me3 establishment, supporting a read-write mechanism in timely chromatin restoration. Finally, we provide a detailed, quantitative picture of the mutual antagonism between K27 and K37 methylation, and propose that this antagonism enhance the stability of epigenetic states across cell division.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:51.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Voelker-Albert
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monomethylated residue; 6x(13)C labeled residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-29 05:43:53	ID requested
1	2024-10-07 13:31:08	announced
⏵ 2	2024-10-22 04:58:59	announced	2024-10-22: Updated project metadata.

Alabert C, Loos C, Voelker-Albert M, Graziano S, Forn, é I, Reveron-Gomez N, Schuh L, Hasenauer J, Marr C, Imhof A, Groth A, Domain Model Explains Propagation Dynamics and Stability of Histone H3K27 and H3K36 Methylation Landscapes. Cell Rep, 30(4):1223-1234.e8(2020) [pubmed]

10.1016/j.celrep.2019.12.060;

submitter keyword: Q-Exactive HF, Chromatin domains,Histone modifications, Data modeling

Prof. Dr. Axel Imhof
contact affiliation	Biomedical Center, Chromatin Proteomics Group, Department of Molecular Biology, Faculty of Medicine, Ludwig-Maximilians-Universität München, Großhaderner Strasse 9, 82152 Planegg-Martinsried, Germany.
contact email	imhof@lmu.de
lab head
Moritz Voelker-Albert
contact affiliation	LMU Munich
contact email	Moritz.Voelker-Albert@med.uni-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014807
     1. Label: PRIDE project
     2. Name: Domain model explains propagation dynamics and stability of K27 and K36 methylation landscape