PXD014790

PXD014790 is an original dataset announced via ProteomeXchange.

Title	Surfaceome dynamics and surface trafficking during neuronal development and synaptic plasticity
Description	Surface proteins are of fundamental importance for formation of synaptic connections and activity-dependent plasticity. Here, we used a spatiotemporally resolved cell-surface proteotype analysis to characterize the neuronal surface-exposed proteome, or surfaceome, during neuronal development and synapse formation in primary neuronal cultures. We established a map of the neuronal surfaceome, which includes about 1,000 surface proteins, and analyzed the dynamic remodeling of the quantitative surfaceome during development. We identified time-resolved surface-abundance profile clusters that correspond to distinct stages of neuronal development. We discovered that surface abundance changes can correlate with or be uncoupled from the total cellular abundance. Finally, we observed system-wide surfaceome modulation in response to homeostatic synaptic scaling and exocytosis of diverse cargo during long-term potentiation.
HostingRepository	PRIDE
AnnounceDate	2020-10-05
AnnouncementXML	Submission_2020-10-19_00:53:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc van Oostrum
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	deaminated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-29 01:37:27	ID requested
1	2020-10-05 06:02:08	announced
⏵ 2	2020-10-19 00:53:14	announced	2020-10-19: Updated publication reference for PubMed record(s): 33020478.

van Oostrum M, Campbell B, Seng C, M, ü, ller M, Tom Dieck S, Hammer J, Pedrioli PGA, F, ö, ldy C, Tyagarajan SK, Wollscheid B, Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity. Nat Commun, 11(1):4990(2020) [pubmed]

submitter keyword: Neuroproteomics, surfaceome, synapse, spatial, surface

Bernd Wollscheid
contact affiliation	Biomedical Proteomics Platform, Department of Health Sciences and Technology, ETH Zurich, 8093 Zurich, Switzerland Institute of Molecular Systems Biology at the Department of Biology, ETH Zurich, Zurich 8093, Switzerland
contact email	wbernd@ethz.ch
lab head
Marc van Oostrum
contact affiliation	ETH Zürich
contact email	marcv@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD014790

PRIDE project URI

• PRIDE
  1. PXD014790
     1. Label: PRIDE project
     2. Name: Surfaceome dynamics and surface trafficking during neuronal development and synaptic plasticity