PXD014774

PXD014774 is an original dataset announced via ProteomeXchange.

Title	Widespread glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens
Description	Carbon catabolite repression (CCR) occurs in the presence of sufficient concentrations of easy metabolizable carbon sources (e.g. glucose), down-regulating the expression of genes encoding enzymes involved in the breakdown of complex carbon sources. The extent of CCR at a global level is unknown in wood-rotting fungi – and was investigated in Dichomitus squalens with transcriptomics and exo-proteomics. Approximately 10% of expressed genes had lower expression in presence of glucose compared to Avicel or xylan alone. The glucose repressed genes included key components for utilization of plant biomass – CAZymes, sugar transporters and carbon catabolic genes. The majority of polysaccharide degrading CAZymes were repressed and included activities towards all major polymers. The repression found in the transcriptome was strongly supported by exo-proteomics – there was repression of almost all of the CAZymes whose transcripts were repressed on Avicel. The clear CCR-mediated effects indicate biotech production of CAZymes by D. squalens would benefit from de-repressed or constitutively expressing strains. The extent of CCR is surprising given that D. squalens rarely experiences high sugar concentrations in its woody environment.
HostingRepository	PRIDE
AnnounceDate	2019-10-07
AnnouncementXML	Submission_2019-10-25_06:02:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014774
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marcos Di Falco
SpeciesList	scientific name: Dichomitus squalens; NCBI TaxID: 114155;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-26 03:50:39	ID requested
1	2019-10-07 01:41:34	announced
⏵ 2	2019-10-25 06:02:26	announced	2019-10-25: Updated publication reference for PubMed record(s): 31585998.

Daly P, Peng M, Di Falco M, Lipzen A, Wang M, Ng V, Grigoriev IV, Tsang A, M, ä, kel, ä MR, de Vries RP, . Appl Environ Microbiol, 85(23):(2019) [pubmed]

submitter keyword: Dichomitus, carbon catabolite repression, CAZymes, regulation

Ronald P. de Vries
contact affiliation	Westerdijk Fungal Biodiversity Institute, The Netherlands
contact email	r.devries@wi.knaw.nl
lab head
Marcos Di Falco
contact affiliation	Concordia University
contact email	marcos.difalco@concordia.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014774
     1. Label: PRIDE project
     2. Name: Widespread glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens