PXD014771

PXD014771 is an original dataset announced via ProteomeXchange.

Title	Customizing functionalized cofactor mimics to study the human pyridoxal 5'-phosphate-binding proteome
Description	Here we apply functionalized cofactor derivatives to study vitamin B6-dependent enzymes in human cells. We are able to cover for a large fraction of the pyridoxal 5'-phosphate-binding proteome (PLP-ome) and validate our results exemplarily for two PLP-DEs. One of those, named pyridoxal 5'-phosphate binding protein (PLPBP) is characterized further including studies with chemical cross-linking combined with mass spectrometry. We proceed with an in situ PLP-DE target screen applying B6-targeting compounds revealing interesting insights into selectivity. Finally, we broaden our analysis to multiple human cell lines revealing an altered PLP-DE target profile.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:40.514.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anja Fux
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N6-pyridoxal phosphate-L-lysine
Instrument	Orbitrap Fusion ETD; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-26 02:28:35	ID requested
1	2019-08-27 08:19:10	announced
⏵ 2	2024-10-22 04:58:42	announced	2024-10-22: Updated project metadata.

Fux A, Pfanzelt M, Kirsch VC, Hoegl A, Sieber SA, Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5'-Phosphate-Binding Proteome. Cell Chem Biol, 26(10):1461-1468.e7(2019) [pubmed]

10.1016/j.chembiol.2019.08.003;

submitter keyword: pyridoxal-probe, vitamin B6 antagonist,Pyridoxal 5'-phosphate

Stephan Axel Sieber
contact affiliation	Chair of Organic Chemistry II Department of Chemistry Technical University Munich 85748 Garching
contact email	stephan.sieber@tum.de
lab head
Anja Fux
contact affiliation	Techincal University Munich
contact email	anja.fux@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD014771

PRIDE project URI

• PRIDE
  1. PXD014771
     1. Label: PRIDE project
     2. Name: Customizing functionalized cofactor mimics to study the human pyridoxal 5'-phosphate-binding proteome