PXD014765

PXD014765 is an original dataset announced via ProteomeXchange.

Title	Approaching absolute quantification using label-free approaches and external proteins standards in yeast cultures
Description	Protein extracts of Saccharomyces cerevisiae CEN.PK113-7D cultivated in chemostats under different conditions. Representative samples containing aliquots of all conditions were spiked with UPS2 standard (Sigma) to estimate absolute values in fmol. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: T2- Standard condition : 30°C, pH 5.5 T3- High temperature: 36°C, pH 5.5 T4- Low pH: 30°C, pH 3.5 T5- Osmotic stress : 30°C, pH 5.5, 1M KCl T6- Anaerobic condition Furthermore, representative samples pooling aliquots of each condition are indicated as "bulk" samples. These samples were spiked with UPS proteins. A validation step was carried out by spiking 4 external proteins at known concentrations within the yeast and UPS proteins mixture
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:48:52.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aaron Millan Oropeza
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	alkylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-25 05:22:20	ID requested
⏵ 1	2022-02-15 09:48:53	announced

Mill, á, n-Oropeza A, Blein-Nicolas M, Monnet V, Zivy M, Henry C, Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes, 10(1):(2022) [pubmed]

submitter keyword: Absolute quantification, Chemostats, Saccharomyces, UPS, Yeast

Celine Henry
contact affiliation	Micalis Institute, PAPPSO, INRA, AgroParisTech, Université Paris-Saclay, 78350, Jouy-en-Josas, France
contact email	celine.henry@inra.fr
lab head
Aaron Millan Oropeza
contact affiliation	INRA
contact email	aaron.millan-oropeza@inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014765
     1. Label: PRIDE project
     2. Name: Approaching absolute quantification using label-free approaches and external proteins standards in yeast cultures