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PXD014760

PXD014760 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS based comparative proteomics of floral nectars reveal different mechanisms involved in floral defense of Nicotiana spp. P. hybrida and D. stramonuim
DescriptionFloral nectar is a rich secretion produced by nectaries offered as an attractive reward to pollinators. Floral nectaries secrete an aqueous solution largely composed of sugars and amino acids as well as an array of proteins called nectarins. These provide one of the main mechanisms of floral defense in Nicotiana nectar. Due to its composition, floral nectar provides an ideal source of nutrients for specialized microorganisms that can change the nectar composition. Understanding of floral defenses and the role of nectar proteins is essential to predict the impacts in microbial growth, nectar composition, and relationships between plants and pollinators. In order to deepen knowledge about Nicotiana spp. nectar proteins, we used LC-MS/MS-based comparative proteomic analysis to identify 22 proteins from Petunia hybrida, 35 proteins from Datura stramonium, and 144 proteins from 23 different species of Nicotiana. GO enrichment analysis and secretory signal peptide prediction demonstrated that defense/stress was the largest group of proteins in nectars with differential abundance and distribution throughout genus Nicotiana. The Nicotiana spp. proteome consisted of 105 exclusive proteins such as lipid transfer proteins (LTPs), Nectar Redox Cycle proteins, proteases inhibitors (PI), and PR-proteins. Analysis by taxonomic sections demonstrated that LTPs were the most abundant group of proteins in the nectar of Undulatae and Noctiflora sections while nectarins were more abundant proteins in Rusticae, Suaveolens, Polydicliae, and Alata sections. Peroxidases (Pox) and chitinases (Chit) were exclusive to P. hybrida nectar, while D. stramonium proteome had only seven unique proteins. Significant differences were identified between the proteome of taxonomic sections providing relevant insights into the group of proteins related to defense/stress associated with Nectar Redox Cycle, antimicrobial proteins and signaling pathways. The activity of floral nectar proteins is suggested impact the microbial growth. The knowledge about these proteomes provides significant insights into the diversity of proteins secreted in the nectars and the array of mechanisms used by Nicotiana spp. in its floral defense.
HostingRepositoryPRIDE
AnnounceDate2020-01-08
AnnouncementXMLSubmission_2020-01-07_16:13:20.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD014760
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFredy Silva
SpeciesList scientific name: Nicotiana forgetiana; NCBI TaxID: 118696; scientific name: Nicotiana langsdorffii; NCBI TaxID: 118700; scientific name: Nicotiana africana; NCBI TaxID: 200302; scientific name: Nicotiana x sanderae; NCBI TaxID: 200298; scientific name: Nicotiana paniculata; NCBI TaxID: 62141; scientific name: Nicotiana noctiflora; NCBI TaxID: 118705; scientific name: Nicotiana bonariensis; NCBI TaxID: 118694; scientific name: Nicotiana alata; NCBI TaxID: 4087; scientific name: Datura stramonium; NCBI TaxID: 4076; scientific name: Nicotiana quadrivalvis; NCBI TaxID: 278839; scientific name: Nicotiana glutinosa; NCBI TaxID: 35889; scientific name: Nicotiana plumbaginifolia; NCBI TaxID: 4092; scientific name: Nicotiana tabacum; NCBI TaxID: 4097; scientific name: Nicotiana longiflora; NCBI TaxID: 118702; scientific name: Nicotiana glauca; NCBI TaxID: 4090; scientific name: Nicotiana sylvestris; NCBI TaxID: 4096; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Nicotiana gossei; NCBI TaxID: 118698; scientific name: Nicotiana rustica; NCBI TaxID: 4093; scientific name: Nicotiana clevelandii; NCBI TaxID: 81866; scientific name: Nicotiana repanda; NCBI TaxID: 76336; scientific name: Nicotiana maritima; NCBI TaxID: 200308; scientific name: Nicotiana benavidesii; NCBI TaxID: 118691; scientific name: Petunia x hybrida; NCBI TaxID: 4102;
ModificationListDeamidated; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-25 03:36:50ID requested
12020-01-07 16:13:22announced
Publication List
Silva F, Guirgis A, von Aderkas P, Borchers CH, Thornburg R, LC-MS/MS based comparative proteomics of floral nectars reveal different mechanisms involved in floral defense of Nicotiana spp., Petunia hybrida and Datura stramonium. J Proteomics, 213():103618(2020) [pubmed]
Keyword List
submitter keyword: LC-MS/MS, Nicotiana, Nectar, Floral defense, Nectar Redox Cycle
Contact List
Robert Thornburg
contact affiliationIowa State University - Department of Biochemistry Biophysics and Molecular Biology
contact emailthorn@iastate.edu
lab head
Fredy Silva
contact affiliationIowa State University
contact emailfredydavi@gmail.com
dataset submitter
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