PXD014754

PXD014754 is an original dataset announced via ProteomeXchange.

Title	C1R.A*01:01, C1R.A*02:01, C1R.A*24:02 MHC class I immunopeptidome LC-MSMS
Description	Characterisation of peptide ligands of Major histocompatibility class (MHC) I isolated by immunoaffinity purification from the C1R (Class I reduced) B-lymphoblastoid cell line, transfected with the MHC class I allele HLA-A*01:01, or HLA-A*02:01, HLA-A*24:02. In addition, public mass spectrometry (MS) datasets of HLA-I and HLA-II immunopeptidome derived from patients’ samples, PBMC or cell lines, and shotgun proteomics from trypsin/elastase digestion were analysed.
HostingRepository	PRIDE
AnnounceDate	2020-07-27
AnnouncementXML	Submission_2020-07-26_23:13:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shutao Mei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-25 02:56:27	ID requested
1	2020-05-04 01:35:30	announced
⏵ 2	2020-07-26 23:13:14	announced	2020-07-27: Updated publication reference for PubMed record(s): 32357974.

Mei S, Ayala R, Ramarathinam SH, Illing PT, Faridi P, Song J, Purcell AW, Croft NP, Immunopeptidomic Analysis Reveals That Deamidated HLA-bound Peptides Arise Predominantly from Deglycosylated Precursors. Mol Cell Proteomics, 19(7):1236-1247(2020) [pubmed]

submitter keyword: Deamidation, HLA, mass spectromtry, ERAD

Anthony W. Purcell
contact affiliation	Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia (lab head) (lab head)
contact email	anthony.purcell@monash.edu
lab head
Shutao Mei
contact affiliation	Monash University
contact email	shutao.mei@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD014754

PRIDE project URI

• PRIDE
  1. PXD014754
     1. Label: PRIDE project
     2. Name: C1R.A*01:01, C1R.A*02:01, C1R.A*24:02 MHC class I immunopeptidome LC-MSMS