PXD014751 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomics of liver and plasma on the CDAA rat NASH model |
Description | Nonalcoholic steatohepatitis (NASH) is a major cause of liver fibrosis with increasing prevalence worldwide. Currently there are no approved drugs available. The development of new therapies is difficult as diagnosis and staging requires biopsies. Consequently, predictive plasma biomarkers would be useful for drug development. To identify new plasma biomarkers in a choline-deficient L-amino acid-defined diet rat NASH model we analyzed liver and plasma samples by proteomics, revealing disease relevant signatures and a high correlation between liver mRNA and protein changes. We developed a multi-dimensional attribute ranking approach integrating multi-omics data with liver histology and prior knowledge uncovering known human markers, but also novel candidates. Using regression analysis, we show that the top-ranked plasma markers were highly predictive for fibrosis in our model and hence can serve as preclinical plasma biomarkers. Our approach presented here illustrates the power of multi-omics analyses combined with plasma proteomics and is readily applicable to human biomarker discovery. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:58:40.649.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniel Veyel |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-25 01:57:03 | ID requested | |
1 | 2020-01-28 04:36:31 | announced | |
2 | 2020-02-04 02:28:03 | announced | 2020-02-04: Updated publication reference for PubMed record(s): 31992752. |
3 | 2020-02-17 03:22:41 | announced | 2020-02-04: Updated publication reference for PubMed record(s): 31992752.
2020-02-17: Updated publication reference for PubMed record(s): 31992752. |
⏵ 4 | 2024-10-22 04:58:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41598-020-58030-6; |
Veyel D, Wenger K, Broermann A, Bretschneider T, Luippold AH, Krawczyk B, Rist W, Simon E, Biomarker discovery for chronic liver diseases by multi-omics - a preclinical case study. Sci Rep, 10(1):1314(2020) [pubmed] |
Keyword List
submitter keyword: LC-MSMS, Plasma,Han Wistar rat, Liver |
Contact List
Wolfgang Rist |
contact affiliation | Boehringer Ingelheim Pharma GmbH & Co. KG, Drug Discovery Sciences |
contact email | wolfgang.rist@boehringer-ingelheim.com |
lab head | |
Daniel Veyel |
contact affiliation | Boehringer Ingelheim Pharma GmbH & Co. KG |
contact email | daniel.veyel@boehringer-ingelheim.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014751
- Label: PRIDE project
- Name: Comparative proteomics of liver and plasma on the CDAA rat NASH model