PXD014748

PXD014748 is an original dataset announced via ProteomeXchange.

Title	Effects of simulated space radiations on the tomato root proteome
Description	Plant cultivation on spacecraft or planetary outposts is a promising and actual perspective both for food and bioactive molecules production. To this aim, plants response to ionizing radiations, as an important component of space radiation, must be assessed through on-ground experiments due to the potentially fatal effects on living systems. Hereby we investigated the effects of X-rays and -rays exposure on tomato ‘hairy root’ cultures (HRCs), which represent a solid platform for the production of pharmaceutically relevant molecules, including metabolites and recombinant proteins. In a space application perspective, we used a HRC system previously fortified through the accumulation of anthocyanins, which are known for their anti-oxidant properties. Roots were independently exposed to different photon radiations, namely X-rays (250 kV) and -rays (Co60, 1.25 MeV), both at the intensity of 0.5, 5 and 10 Gy. Molecular changes induced in the proteome of HRCs were investigated by a comparative approach based on Two-Dimensional Difference In-Gel electrophoresis (2D-DIGE) technology and mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2019-09-25
AnnouncementXML	Submission_2019-11-12_23:37:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	anna maria salzano
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-25 01:39:32	ID requested
1	2019-09-25 06:37:21	announced
⏵ 2	2019-11-12 23:37:56	announced	2019-11-13: Updated publication reference for PubMed record(s): 31708949.

Desiderio A, Salzano AM, Scaloni A, Massa S, Pimpinella M, De Coste V, Pioli C, Nardi L, Benvenuto E, Villani ME, Effects of Simulated Space Radiations on the Tomato Root Proteome. Front Plant Sci, 10():1334(2019) [pubmed]

submitter keyword: Tomato, space, radiation, 2DIGE

Andrea Scaloni
contact affiliation	Istituto per il Sistema Produzione Animale in Ambiente (ISPAAM) , Consiglio Nazionale delle Ricerche ITALY
contact email	andrea.scaloni@ispaam.cnr.it
lab head
anna maria salzano
contact affiliation	CNR ISPAAM
contact email	annamaria.salzano@cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014748

PRIDE project URI

• PRIDE
  1. PXD014748
     1. Label: PRIDE project
     2. Name: Effects of simulated space radiations on the tomato root proteome