PXD014743

PXD014743 is an original dataset announced via ProteomeXchange.

Title	Identification of zein proteome by multi-enzyme digestion
Description	Zeins are a class of prolamine proteins extracted from maize, and are extensively used in the food and pharmaceutical industries. Therefore, characterization of its components is essential for quality control and safety evaluation. However, zein proteins have low abundance of the trypsin targets lysine and arginine, thereby requiring enzymes of different cleavage sites for shotgun proteomic analyses. To this end, we performed in silico digestion of zein proteins using tandem combinations of trypsin/Lys-C, trypsin/chymotrypsin and trypsin/thermolysin, in order to improve protein sequence coverage and subsequent identification by LC-MS/MS analysis. Trypsin/chymotrypsin yielded the highest protein sequence coverage of up to 79.5% by the filter-aided sample preparation (FASP) method, and increased the number of detectable proteins from 11 to 35 compared to trypsin/Lys-C. Furthermore, SDS-PAGE revealed 37 proteins in the zein extract, as well as the possibility of protein polymers. A total of 51 proteins were detected by FASP and in-gel digestion, including 37 zein and 14 non-zein proteins. In addition, 420 peptides originating from 71 zein proteins were identified, of which 116 were predicted as bioactive. In conclusion, in silico digestion coupled with multi enzyme digestion can significantly improve the proteomic and peptidomic analysis of complex protein extracts.
HostingRepository	iProX
AnnounceDate	2019-07-25
AnnouncementXML	Submission_2021-01-08_05:34:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Menglin Li
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-25 00:46:51	ID requested
1	2019-07-25 00:56:19	announced
⏵ 2	2021-01-08 05:34:53	announced	2021-01-08: Update publication information

Li M, Zheng H, Lin M, Zhu W, Zhang J, Characterization of the protein and peptide of excipient zein by the multi-enzyme digestion coupled with nano-LC-MS/MS. Food Chem, 321():126712(2020) [pubmed]

submitter keyword: Excipient zein, In silicon digestion, Multi enzyme digestion, Proteomics, Peptidomics

Jinlan Zhang
contact affiliation	Institute of Meteria Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	zhjl@imm.ac.cn
lab head
Menglin Li
contact affiliation	Institute of Meteria Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	menglinli86@gmail.com
dataset submitter

iProX dataset URI