PXD014733

PXD014733 is an original dataset announced via ProteomeXchange.

Title	Lysosomal proteomics by affinity purification-mass spectrometry
Description	The aims of this project is to establish a comprehensive proteomic map of the lysosomes using isolated lysosomes via affinity purification. Affinity-purified mitochondria was used as a control to demonstrate the organelle specificity. In this study, we identified several lipid transporters that are associated with the lysosome and further characterized their roles in sterol-dependent mTORC1 regulation.
HostingRepository	PRIDE
AnnounceDate	2019-09-27
AnnouncementXML	Submission_2019-09-27_06:52:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014733
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chun-Yan Lim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-24 06:49:42	ID requested
⏵ 1	2019-09-27 06:52:51	announced

Lim CY, Davis OB, Shin HR, Zhang J, Berdan CA, Jiang X, Counihan JL, Ory DS, Nomura DK, Zoncu R, ER-lysosome contacts enable cholesterol sensing by mTORC1 and drive aberrant growth signalling in Niemann-Pick type C. Nat Cell Biol, 21(10):1206-1218(2019) [pubmed]

submitter keyword: Lysosome immunoprecipitation, mTORC1, organelle

Roberto Zoncu
contact affiliation	Assistant Professor of Biochemistry, Biophysics and Structural Biology Molecular and Cell Biology Department University of California at Berkeley Li Ka Shing Center, room 300A Berkeley, CA 94720 rzoncu@berkeley.edu
contact email	rzoncu@berkeley.edu
lab head
Chun-Yan Lim
contact affiliation	UC Berkeley
contact email	chunyan_lim@berkeley.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014733

PRIDE project URI

• PRIDE
  1. PXD014733
     1. Label: PRIDE project
     2. Name: Lysosomal proteomics by affinity purification-mass spectrometry