PXD014716

PXD014716 is an original dataset announced via ProteomeXchange.

Title	Effects of PGRMC1 phosphorylation status on MIA PaCa-2 cells
Description	Progesterone Receptor Membrane Component 1 (PGRMC1) is expressed in many cancer cells, where it is associated with detrimental patient outcomes. Multiple different functions and cellular locations have been attributed to PGRMC1 in a variety of contexts, however the mechanisms underlying PGRMC1 biology remain obscure. The protein contains several phosphorylated residues including tyrosines which were acquired early in animal evolution and could be involved with animal cell differentiation mechanisms. Here we demonstrate that mutagenic manipulation of PGRMC1phosphorylation status in MIA PaCa-2 (MP) pancreatic cells exerts broad pleiotropic effects, influencing cell plasticity and tumorigenicity, as assayed by cell biological and proteomics measurements. Relative to MP cells over-expressing hemagglutinin (HA)-tagged wild-type PGRMC1- HA (WT), cells expressing a PGRMC1-HA-S57A/S181A double mutant (DM) exhibited reduced levels of proteins involved in energy metabolism and mitochondrial function. This was associated with Rho-kinase inhibitor (ROCKI)-sensitive changes including altered cell shape, motility, increased PI3K/Akt and JNK activity, and fragmented mitochondrial morphology. An S57A/Y180F/S181A triple mutant (TM) reduced PI3K/Akt and JNK activation, indicating involvement of Y180. Both TM cells and Y180F single mutant cells exhibited attenuated mouse xenograft tumor growth. Tyrosine 180 phosphorylation status of PGRMC1 exerts dramatic influence over cancer cell biology.
HostingRepository	PRIDE
AnnounceDate	2020-03-03
AnnouncementXML	Submission_2020-05-27_04:38:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dana Pascovici
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-23 05:51:44	ID requested
1	2020-03-03 01:51:54	announced
⏵ 2	2020-05-27 04:38:30	announced	2020-05-27: Updated publication reference for PubMed record(s): 32245408.

Thejer BM, Adhikary PP, Kaur A, Teakel SL, Van Oosterum A, Seth I, Pajic M, Hannan KM, Pavy M, Poh P, Jazayeri JA, Zaw T, Pascovici D, Ludescher M, Pawlak M, Cassano JC, Turnbull L, Jazayeri M, James AC, Coorey CP, Roberts TL, Kinder SJ, Hannan RD, Patrick E, Molloy MP, New EJ, Fehm TN, Neubauer H, Goldys EM, Weston LA, Cahill MA, PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. BMC Mol Cell Biol, 21(1):24(2020) [pubmed]

submitter keyword: proteomics, SWATH, pancreatic cancer, phosphorylation

Michael A. Cahill
contact affiliation	School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650
contact email	mcahill@csu.edu.au
lab head
Dana Pascovici
contact affiliation	Australian Proteome Analysis Facility
contact email	dana.pascovici@mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD014716

PRIDE project URI

• PRIDE
  1. PXD014716
     1. Label: PRIDE project
     2. Name: Effects of PGRMC1 phosphorylation status on MIA PaCa-2 cells