PXD014695

PXD014695 is an original dataset announced via ProteomeXchange.

Title	α-arrestin-dependent endocytosis after cycloheximide treatment
Description	To identify new plasma membrane (PM) proteins that require α-arrestins for endocytosis, we performed an iTRAQ-based quantitative proteomic screening. We analysed and compared the PM proteome for each arrestin knockout (art∆) strain to a WT strain after a 90 min cycloheximide (CHX) treatment. CHX is a translation inhibitor and can trigger the endocytosis of several transporters in a substrate-independent manner. We searched for proteins that underwent CHX-induced endocytosis in a WT strain but that remained stabilized at the PM in an art∆ strain.
HostingRepository	PRIDE
AnnounceDate	2019-09-25
AnnouncementXML	Submission_2019-09-25_11:51:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jérôme Savocco
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-22 06:46:27	ID requested
⏵ 1	2019-09-25 11:51:32	announced

Dataset with its publication pending

submitter keyword: endocytosis, arrestin, cycloheximide, plasma membrane

Pierre Morsomme
contact affiliation	Louvain Institute of Biomolecular Science and Technology (LIBST), UCLouvain, Belgium
contact email	pierre.morsomme@uclouvain.be
lab head
Jérôme Savocco
contact affiliation	UCLouvain
contact email	jerome.savocco@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014695

PRIDE project URI

• PRIDE
  1. PXD014695
     1. Label: PRIDE project
     2. Name: α-arrestin-dependent endocytosis after cycloheximide treatment