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PXD014689

PXD014689 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStress response and detoxification mechanisms involved in non-target site herbicide resistance in sunflower
DescriptionThe objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in Imisun sunflower resistance. Two sunflower inbred lines were used: HA 425 and HA 89, imidazolinone (IMI)-resistant and susceptible, respectively. Growth response to imazethapyr herbicide in combination with P450s inhibitors 1-aminobenzotriazole (ABT) and piperonyl butoxide (PBO) was evaluated in 15-day-old sunflower plantlets. Roots were collected and label-free quantitation (LFQ) proteomic analysis were carried out in order to characterize plant response to IMI. Roots of 15-day-old plants treated with 0 and 3.3 µM imazethapyr were frozen in liquid nitrogen. Protein extraction was performed as described in Wu et al. (2014) from 1 g root tissue per genotype/treatment combination.
HostingRepositoryPRIDE
AnnounceDate2020-03-25
AnnouncementXMLSubmission_2020-03-25_06:08:39.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTatiana Vega
SpeciesList scientific name: Helianthus annuus; NCBI TaxID: 4232;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-22 05:48:10ID requested
12020-03-25 06:08:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Helianthus annuus, herbicide resistance, detoxification, imidazolinone, NTSR
Contact List
Tatiana A. Vega
contact affiliationIICAR, Instituto de Investigaciones en Ciencias Agrarias, Facultad de Ciencias Agrarias, UNR-CONICET. Campo Experimental “José Villarino”, S2125ZAA Zavalla, ARGENTINA
contact emailtvega@unr.edu.ar
lab head
Tatiana Vega
contact affiliationIICAR-CONICET/UNR
contact emailtvega@unr.edu.ar
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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