PXD014689

PXD014689 is an original dataset announced via ProteomeXchange.

Title	Stress response and detoxification mechanisms involved in non-target site herbicide resistance in sunflower
Description	The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in Imisun sunflower resistance. Two sunflower inbred lines were used: HA 425 and HA 89, imidazolinone (IMI)-resistant and susceptible, respectively. Growth response to imazethapyr herbicide in combination with P450s inhibitors 1-aminobenzotriazole (ABT) and piperonyl butoxide (PBO) was evaluated in 15-day-old sunflower plantlets. Roots were collected and label-free quantitation (LFQ) proteomic analysis were carried out in order to characterize plant response to IMI. Roots of 15-day-old plants treated with 0 and 3.3 µM imazethapyr were frozen in liquid nitrogen. Protein extraction was performed as described in Wu et al. (2014) from 1 g root tissue per genotype/treatment combination.
HostingRepository	PRIDE
AnnounceDate	2020-03-25
AnnouncementXML	Submission_2020-03-25_06:08:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tatiana Vega
SpeciesList	scientific name: Helianthus annuus; NCBI TaxID: 4232;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-22 05:48:10	ID requested
⏵ 1	2020-03-25 06:08:40	announced

Dataset with its publication pending

submitter keyword: Helianthus annuus, herbicide resistance, detoxification, imidazolinone, NTSR

Tatiana A. Vega
contact affiliation	IICAR, Instituto de Investigaciones en Ciencias Agrarias, Facultad de Ciencias Agrarias, UNR-CONICET. Campo Experimental “José Villarino”, S2125ZAA Zavalla, ARGENTINA
contact email	tvega@unr.edu.ar
lab head
Tatiana Vega
contact affiliation	IICAR-CONICET/UNR
contact email	tvega@unr.edu.ar
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014689
     1. Label: PRIDE project
     2. Name: Stress response and detoxification mechanisms involved in non-target site herbicide resistance in sunflower