PXD014688

PXD014688 is an original dataset announced via ProteomeXchange.

Title	A Temporal Study of the Perturbation of Crustacean Neuropeptides Due to Severe Hypoxia Using 4-Plex Reductive Dimethylation
Description	Hypoxia (i.e., low oxygen (O2) levels) is a common environmental challenge for several aquatic species, including fish and invertebrates. To survive or escape these conditions, these animals have developed novel biological mechanisms, some regulated by neuropeptides. By utilizing mass spectrometry, this study aims to provide a global perspective of neuropeptides in the blue crab, Callinectes sapidus, and their changes over time (0, 1, 4, and 8 hours) due to severe hypoxia (~10% O2 water saturation) stress using a 4-plex dimethyl labeling strategy to increase throughput. Using both electrospray ionization and matrix-assisted laser desorption/ionization provided complementary coverage– 88 neuropeptides were identified with only five overlapping between the two ionization techniques. Interesting trends include (1) an overall decrease in expression due to hypoxia exposure, (2) a return to basal levels after 4 or 8 hours of exposure following an initial response, (3) changes only after 4+ hours exposure, and (4) an oscillating pattern. Overall, this study boosts the power of multiplexed quantitation to understand the large-scale changes due to severe hypoxia stress over time.
HostingRepository	PRIDE
AnnounceDate	2020-03-05
AnnouncementXML	Submission_2020-03-05_11:02:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chris Sauer
SpeciesList	scientific name: Callinectes sapidus; NCBI TaxID: 6763;
ModificationList	amidated residue; dehydrated residue; monohydroxylated residue
Instrument	MALDI LTQ Orbitrap; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-22 05:47:16	ID requested
⏵ 1	2020-03-05 11:02:49	announced

Buchberger AR, Sauer CS, Vu NQ, DeLaney K, Li L, Temporal Study of the Perturbation of Crustacean Neuropeptides Due to Severe Hypoxia Using 4-Plex Reductive Dimethylation. J Proteome Res, 19(4):1548-1555(2020) [pubmed]

submitter keyword: Neuropeptides, Isotopic Labeling, MALDI, LC-MS, Hypoxia

Lingjun Li
contact affiliation	School of Pharmacy and Department of Chemistry, University of WI-Madison, Madison, WI, United States
contact email	lingjun.li@wisc.edu
lab head
Chris Sauer
contact affiliation	University of Wisconsin - Madison
contact email	csauer@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014688
     1. Label: PRIDE project
     2. Name: A Temporal Study of the Perturbation of Crustacean Neuropeptides Due to Severe Hypoxia Using 4-Plex Reductive Dimethylation