PXD014679

PXD014679 is an original dataset announced via ProteomeXchange.

Title	A peptidomimetic proprotein convertase inhibitor is a potential anti-glioma drug as revealed by biological assays and proteomic studies
Description	Proprotein convertases (PCs) are proteases which have an important regulatory function in a wide variety of biological processes. However, these enzymes have a key role in the activation of many cancer-related proteins and promotes the malignant phenotype of cancer cells. Here, with proteomics and biological tests, we investigated the consequences of the use of a peptidomimetic PCs inhibitor on glioma cells and macrophages in the same time and its potential application to cancer therapy. Thus, we demonstrated that the PCs inhibitor acts on macrophage activation, characterized by the secretion of pro-inflammatory and anti-tumoral factor. Also, the PCs inhibitor triggers a strong decrease of the proliferation and invasion of glioma cells, even when they are associated with macrophages in spheroids. In fact, the PCs inhibitor induces great changes in the intracellular and secreted protein profiles of the glioma cells. Many of cancerous processes are affected, such as cell growth, proliferation, angiogenesis and the immunosuppressive capacity of cancer cells. Taken together, these data, establish that the inhibitor acts both on cancer cells and macrophages and can be used as a potential anti-cancer therapeutic strategy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:06:27.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soulaimane Aboulouard
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-19 07:22:46	ID requested
1	2020-05-27 03:09:30	announced
⏵ 2	2024-10-22 05:06:33	announced	2024-10-22: Updated project metadata.

10.1016/j.omto.2020.03.005;

Rose M, Duhamel M, Aboulouard S, Kobeissy F, Le Rhun E, Desmons A, Tierny D, Fournier I, Rodet F, Salzet M, The Role of a Proprotein Convertase Inhibitor in Reactivation of Tumor-Associated Macrophages and Inhibition of Glioma Growth. Mol Ther Oncolytics, 17():31-46(2020) [pubmed]

submitter keyword: 3D-culture, LC-MSMS,macrophages, glioma, Proteomic

Michel Salzet
contact affiliation	PRISM - Unité INSERM 1192 Université de Lille France
contact email	michel.salzet@univ-lille.fr
lab head
Soulaimane Aboulouard
contact affiliation	PRISM
contact email	soulaimane.aboulouard@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014679

PRIDE project URI

• PRIDE
  1. PXD014679
     1. Label: PRIDE project
     2. Name: A peptidomimetic proprotein convertase inhibitor is a potential anti-glioma drug as revealed by biological assays and proteomic studies