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PXD014678

PXD014678 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePALB2 ChAM acetylation mapping in human cells
DescriptionThe Chromatin Association Motif (ChAM) of the PArtner and Localizer of BRCA2 (PALB2) is essential for DNA repair function by homologous recombination in human cells. Molecular mechanisms governing ChAM interaction with chromatin remain unexplored. In this study, we have mapped acetylated lysines within ChAM domain, which are contained within a region essential to regulate its association with chromatin. GFP-ChAM sample submitted for proteomics analysis for acetylated lysines mapping was purified from HEK293T transiently expressing GFP-ChAM. GFP-ChAM was purified by GFP pull down using GFP-Trap agarose beads (Chromotek) and washed at 250mM NaCl.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:46:29.647.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarjorie Fournier
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-19 07:20:48ID requested
12023-03-10 13:03:24announced
22023-11-14 07:46:31announced2023-11-14: Updated project metadata.
Publication List
Fournier M, Rodrigue A, Milano L, Bleuyard JY, Couturier AM, Wall J, Ellins J, Hester S, Smerdon SJ, Tora L, Masson JY, Esashi F, KAT2-mediated acetylation switches the mode of PALB2 chromatin association to safeguard genome integrity. Elife, 11():(2022) [pubmed]
Keyword List
submitter keyword: acetylation, DNA repair, chromatin
Contact List
Fumiko Esashi
contact affiliationUniversity of Oxford, Sir William Dunn School of Pathology South Parks Road, Oxford OX1 3RE, UK
contact emailfumiko.esashi@path.ox.ac.uk
lab head
Marjorie Fournier
contact affiliationUniversity of Oxford
contact emailmarjorie.fournier@path.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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