PXD014678 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PALB2 ChAM acetylation mapping in human cells |
Description | The Chromatin Association Motif (ChAM) of the PArtner and Localizer of BRCA2 (PALB2) is essential for DNA repair function by homologous recombination in human cells. Molecular mechanisms governing ChAM interaction with chromatin remain unexplored. In this study, we have mapped acetylated lysines within ChAM domain, which are contained within a region essential to regulate its association with chromatin. GFP-ChAM sample submitted for proteomics analysis for acetylated lysines mapping was purified from HEK293T transiently expressing GFP-ChAM. GFP-ChAM was purified by GFP pull down using GFP-Trap agarose beads (Chromotek) and washed at 250mM NaCl. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:46:29.647.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marjorie Fournier |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-19 07:20:48 | ID requested | |
1 | 2023-03-10 13:03:24 | announced | |
⏵ 2 | 2023-11-14 07:46:31 | announced | 2023-11-14: Updated project metadata. |
Publication List
Fournier M, Rodrigue A, Milano L, Bleuyard JY, Couturier AM, Wall J, Ellins J, Hester S, Smerdon SJ, Tora L, Masson JY, Esashi F, KAT2-mediated acetylation switches the mode of PALB2 chromatin association to safeguard genome integrity. Elife, 11():(2022) [pubmed] |
Keyword List
submitter keyword: acetylation, DNA repair, chromatin |
Contact List
Fumiko Esashi |
contact affiliation | University of Oxford, Sir William Dunn School of Pathology South Parks Road, Oxford OX1 3RE, UK |
contact email | fumiko.esashi@path.ox.ac.uk |
lab head | |
Marjorie Fournier |
contact affiliation | University of Oxford |
contact email | marjorie.fournier@path.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014678
- Label: PRIDE project
- Name: PALB2 ChAM acetylation mapping in human cells