PXD014673

PXD014673 is an original dataset announced via ProteomeXchange.

Title	Integrative Structural Characterization of the Human Canonical and Non-canonical Cop9 Signalosome Complexes
Description	The COP9 signalosome (CSN) is an evolutionarily conserved protein complex that functions as a deneddylase to inactivate Cullin-RING E3 ligases for controlling protein ubiquitination. CSN possesses structural flexibility that is important for its activation upon binding to a diverse array of CRLs. The canonical and non-canonical CSN complexes consist of 8 (CSN1-8) and 9 (CSN1-9) subunits, respectively. Although CSN9 is not essential for CSN assembly and function, it appears to be important in non-catalytic regulation of CRLs by CSN. Here we employed a combinatory cross-linking mass spectrometry (XL-MS) approach to generate the largest PPI maps of human CSN complexes, which significantly enhanced the precision of integrative structural modeling. The resulting integrative structures allowed us not only to elucidate architectures of both complexes, but also to assess CSN structural dynamics that was not described in the crystal structure. In addition, we have determined CSN9 docking sites and its impact on the CSN structure. While CSN9 binding did not induce global conformational changes, it triggered subunit local reorientations that may be associated with CSN9 involvement in CSN-mediated steric regulation of CRLs.
HostingRepository	PRIDE
AnnounceDate	2020-02-04
AnnouncementXML	Submission_2020-02-04_11:54:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Craig Gutierrez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-19 04:06:49	ID requested
⏵ 1	2020-02-04 11:54:52	announced

Dataset with its publication pending

submitter keyword: Cross-linking, Mass Spectrometry, MS-cleavable, Sulfoxided based cross-linkers, Cop9 Signalosme , Intergrative Modeling Platform (IMP)

Lan Huang
contact affiliation	Physiology and Biophysics
contact email	lanhuang@uci.edu
lab head
Craig Gutierrez
contact affiliation	Huang Lab
contact email	cbgutier@uci.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014673
     1. Label: PRIDE project
     2. Name: Integrative Structural Characterization of the Human Canonical and Non-canonical Cop9 Signalosome Complexes