PXD014661

PXD014661 is an original dataset announced via ProteomeXchange.

Title	Identification of Toll-like receptor 4 interacting partner through immunoprecipitation-based chemical cross-linking proteomics
Description	Toll-like receptor 4 (TLR-4) is a transmembrane protein. Its activation leads to NF-kB signaling pathway and proinflammatory cytokines’ production that are responsible for activating innate immunity system. Here, we design a co-immunoprecipitation based cross-linking proteomics approach to reveal the TLR-4 interactome upon treatment of lipopolysaccharides and statin drug in HA-TLR-4 transfected HEK293 cells. A total of 712 differentially expressed proteins were identified and quantified in this study. Selected candidate proteins, macrophage myristoylated alanine-rich C kinase substrate, and creatine kinase, exclusively identified in the treatment of statin along with cross-linkers, were further validated by immunoblotting.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:34:35.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ADA Shahinuzzaman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-18 04:11:54	ID requested
1	2022-10-13 05:36:22	announced
⏵ 2	2023-11-14 07:34:40	announced	2023-11-14: Updated project metadata.

Shahinuzzaman ADA, Kamal AHM, Chakrabarty JK, Rahman A, Chowdhury SM, Identification of Inflammatory Proteomics Networks of Toll-like Receptor 4 through Immunoprecipitation-Based Chemical Cross-Linking Proteomics. Proteomes, 10(3):(2022) [pubmed]

submitter keyword: LPS,HEK293, Statin, TLR, Toll Like Receptor

Saiful M Chowdhury
contact affiliation	Associate Professor, Department of Chemistry and Biochemistry, University of Texas At Arlington
contact email	schowd@uta.edu
lab head
ADA Shahinuzzaman
contact affiliation	University of Texas At Arlington
contact email	ada.shahinuzzaman@mavs.uta.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014661
     1. Label: PRIDE project
     2. Name: Identification of Toll-like receptor 4 interacting partner through immunoprecipitation-based chemical cross-linking proteomics