PXD014660

PXD014660 is an original dataset announced via ProteomeXchange.

Title	Top-Down Mass Spectrometry Analysis of Histone Modifications in Sorghum during Drought Acclimation
Description	This study explores the changes in histone modifications of sorghum bicolor through developmental stages and in response to drought stress in two sorghum genotypes. We analyzed the leaves of 48 plants using top-down mass spectrometry and identified 26 unique histone proteins and 677 unique histone proteoforms. We detected trimethylation on nearly all H2B N-termini where acetylation is commonly expected. In addition, an unexpected modification on H2A histones was assigned to N-pyruvic acid 2-iminylation based on its unique neutral loss of CO2.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:59:08.381.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Sorghum bicolor; NCBI TaxID: 4558;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-18 04:11:38	ID requested
1	2019-10-30 02:32:33	announced
⏵ 2	2024-10-22 03:59:11	announced	2024-10-22: Updated project metadata.

10.1016/j.ymeth.2019.10.007;

Zhou M, Malhan N, Ahkami AH, Engbrecht K, Myers G, Dahlberg J, Hollingsworth J, Sievert JA, Hutmacher R, Madera M, Lemaux PG, Hixson KK, Jansson C, Pa, š, a-Toli, ć L, Top-down mass spectrometry of histone modifications in sorghum reveals potential epigenetic markers for drought acclimation. Methods, 184():29-39(2020) [pubmed]

submitter keyword: post-translational modifications, mass spectrometry, top-down, plant,epigenetics, histone clipping

Ljiljana Pasa-Tolic
contact affiliation	Pacific Northwest National Laboratory, USA
contact email	ljiljana.pasatolic@pnnl.gov
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014660

PRIDE project URI

• PRIDE
  1. PXD014660
     1. Label: PRIDE project
     2. Name: Top-Down Mass Spectrometry Analysis of Histone Modifications in Sorghum during Drought Acclimation