PXD014657

PXD014657 is an original dataset announced via ProteomeXchange.

Title	Palaeoproteomic identification of breast milk protein residues from the archaeological skeletal remains of a neonatal dog
Description	Accurate postmortem estimation of breastfeeding status for archaeological or forensic neonatal remains is difficult. Confident identification of milk-specific proteins associated with these remains, would provide direct evidence of breastmilk consumption. We used liquid chromatography coupled to tandem mass spectrometry (MS) to confidently identify beta-lactoglobulin-1 (LGB1) and whey acidic protein (WAP), major whey proteins associated with a neonatal dog (Canis lupus familiaris) skeleton (430–960 cal AD), from an archaeological site in Hokkaido, Japan. The age at death of the individual was estimated to be approximately two weeks after birth. Protein residues extracted from rib and vertebra fragments were analyzed and identified by matching tandem MS spectra against the dog reference proteome. A total of 200 dog protein groups were detected and at least 1 peptide from canine LGB1 and two peptides from canine WAP were confidently identified. These milk proteins most probably originated from the mother’s breastmilk, ingested by the neonate just before it died. We suggest the milk diffused outside the digestive apparatus during decomposition, and by being absorbed into the bones, it partially preserved. The result of this study suggests that proteomic analysis can be used for postmortem reconstruction of the breastfeeding status at the time of death of neonatal mammalian, by analyzing their skeletal archaeological remains. This method is also applicable to forensic and wildlife studies.
HostingRepository	PRIDE
AnnounceDate	2019-10-10
AnnouncementXML	Submission_2019-10-10_08:44:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takumi Tsutaya
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-18 03:01:25	ID requested
1	2019-09-09 05:23:08	announced
⏵ 2	2019-10-10 08:44:27	announced	2019-10-10: Updated project metadata.

Tsutaya T, Mackie M, Koenig C, Sato T, Weber AW, Kato H, Olsen JV, Cappellini E, Palaeoproteomic identification of breast milk protein residues from the archaeological skeletal remains of a neonatal dog. Sci Rep, 9(1):12841(2019) [pubmed]

submitter keyword: Palaeoproteomics, LC-MS/MS, Dog, Bone

Enrico Cappellini
contact affiliation	Section for Evolutionary Genomics, The GLOBE Institute, University of Copenhagen, Denmark
contact email	ecappellini@bio.ku.dk
lab head
Takumi Tsutaya
contact affiliation	Japan Agency for Marine-Earth Science and Technology
contact email	tsuta16@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014657
     1. Label: PRIDE project
     2. Name: Palaeoproteomic identification of breast milk protein residues from the archaeological skeletal remains of a neonatal dog