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PXD014647

PXD014647 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease
DescriptionLate blight disease (LBD) caused by the pathogen Phytophthora infestans (PI) is the most devastating disease limiting potato production globally. To better understand potato molecular response to LBD, the proteomics and morphological changes of three potato cultivars: Favorita (FA), Mira (MA), and E-malingshu N0.14 (E14) was investigated 5 days after PI inoculation, using iTRAQ-based method. Proteomic analysis of the cultivars resulted in the identification and quantification of a total of 3306 and 2044 proteins. Cluster analysis revealed MA and E14 clustered together separately from FA. Protein expression pattern and related functions revealed the cultivars shared a typical hypersensitive response to PI, including induction of elicitors, oxidative burst, and suppression of photosynthesis. Meanwhile, MA and E14 deployed additional specific responses different from FA, involving high induction of protease inhibitors, serine/threonine kinases, terpenoid, and polyketides, hormone signaling, and transport, which contributed to MA tolerance of LBD. Furthermore, the induction of pathogenesis-related proteins, LRR receptor-like kinases, mitogen-activated protein kinase, WRKY transcription factors, jasmonic acid, and phenolic compounds mediate E14 resistance against LBD. These proteins were confirmed at the transcription level by qPCR and at the translation level by western-blot. Our results provide further insights into the different potato proteomic defense machinery against LBD.
HostingRepositoryiProX
AnnounceDate2019-07-18
AnnouncementXMLSubmission_2021-01-08_04:21:01.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQian Zhou
SpeciesList scientific name: Solanum tuberosum; NCBI TaxID: 4113;
ModificationListiTRAQ8plex reporter+balance reagent acylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-17 23:14:15ID requested
12019-07-17 23:28:31announced
12019-07-17 23:28:31announced
22020-05-25 15:30:00announced2020-05-26: Update information
32021-01-08 04:21:02announced2020-05-26: Update information
2021-01-08: Update publication information.
Publication List
Xiao C, Huang M, Gao J, Wang Z, Zhang D, Zhang Y, Yan L, Yu X, Li B, Shen Y, Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease. BMC Genomics, 21(1):880(2020) [pubmed]
Keyword List
submitter keyword: Potato proteomics, Phytophthora infestans, Late blight disease, Resistant, Susceptible, Hypersensitive response.
Contact List
Chunfang Xiao
contact affiliationHu Bei province,enshi Acandemy of Agricutural sciences
contact emailxiaochunfang22@163.com
lab head
Qian Zhou
contact affiliationShanghai Omicsspace Biotech Co., Ltd.
contact emailzhouqian@omicsspace.com
dataset submitter
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iProX dataset URI